Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry

被引：22

作者：

Popot, Marie-Agnes ^{[1
]}

Woolfitt, Adrian R. ^{[2
]}

Garcia, Patrice ^{[1
]}

Tabet, Jean-Claude ^{[3
]}

机构：

[1] Lab Courses Hippiques, F-91370 Verrieres Le Buisson, France

[2] Ctr Dis Control & Prevent, Atlanta, GA 30341 USA

[3] Univ Paris 06, CNRS, UMR 7613, Grp Spectrometrie Masse,Struct & Fonct Mol Bio Ac, F-75225 Paris 05, France

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 390卷 / 07期

关键词：

bioanalytical methods; biological samples; mass spectrometry;

D O I：

10.1007/s00216-008-1889-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The insulin-like-growth factor (IGF-I) peptide is considered to be the main indirect marker for growth hormone administration (GH) in a horse. Further to a previous investigation on measurement of IGF-I in plasma samples by mass spectrometry, this study focuses on quantitative and qualitative analysis of intact IGF-I in horse plasma. First, protein-transposing software has been developed for IGF-I to facilitate its quantification by HPLC-electrospray-ion-trap mass spectrometry. Second, product-ion scan experiments on IGF-I have been conducted on standard samples, non-fortified equine plasma samples, fortified plasma samples, and equine GH post-administration samples. This "top-down" approach method enables characterisation of fragment ions corresponding to the carboxy terminal end, which can be useful for the confirmation of the presence of IGF-I in plasma samples.

引用

页码：1843 / 1852

页数：10

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