Use of monolithic supports for high-throughput protein and peptide separation in proteomics

被引：21
作者：
Andjelkovic, Uros [1 ,2 ]
Tufegdzic, Srdjan [1 ]
Popovic, Milica [3 ]
机构：
[1] Univ Belgrade, Inst Chem Technol & Met, Dept Chem, Studentski Trg 12-16, Belgrade 11000, Serbia
[2] Univ Rijeka, Dept Biotechnol, Rijeka, Croatia
[3] Univ Belgrade, Fac Chem, Belgrade, Serbia
来源：
ELECTROPHORESIS | 2017年 / 38卷 / 22-23期
关键词：
High-throughput; Liquid chromatography; Mass spectrometry; Monoliths; Proteomics; PERFORMANCE LIQUID-CHROMATOGRAPHY; POROUS POLYMER MONOLITHS; IONIZATION MASS-SPECTROMETRY; EFFICIENT SAMPLE PREPARATION; IMMOBILIZED ENZYME REACTORS; SILICA CAPILLARY COLUMNS; HUMAN SERUM-ALBUMIN; AFFINITY-CHROMATOGRAPHY; INTACT PROTEINS; BOTTOM-UP;
D O I：
10.1002/elps.201700260
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.
引用
收藏
页码：2851 / 2869
页数：19
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