Sensing Glucose in Urine and Serum and Hydrogen Peroxide in Living Cells by Use of a Novel Boronate Nanoprobe Based on Surface-Enhanced Raman Spectroscopy

被引：129

作者：

Gu, Xin ^{[1
]}

Wang, Hao ^{[1
]}

Schultz, Zachary D. ^{[1
]}

Camden, Jon P. ^{[1
]}

机构：

[1] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 14期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

QUANTUM DOTS; CHOLESTEROL BIOSENSOR; FLUORESCENT-PROBE; IN-VIVO; SCATTERING; NANOPARTICLES; MOLECULES; OXIDASE; SUPEROXIDE; STRATEGIES;

D O I：

10.1021/acs.analchem.6b01378

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hydrogen peroxide (H2O2) is known as a key molecule in a variety of biological processes, as well as a crucial byproduct in many enzymatic reactions. Therefore, being able to selectively and sensitively detect H2O2 is not only important in monitoring, estimating, and decoding H2O2 relevant physiological pathways but also very helpful in developing enzymatic-based biosensors for other analytes of interest. Herein, we report a plasmonic probe for H2O2 based on 3-mercaptophenylboronic acid (3-MPBA) modified gold nano particles (AuNPs) which is coupled with surface-enhanced Raman scattering (SERS) to yield a limit of detection (LOD) of 70 nM. Our probe quantifies both exogenous and endogenous H2O2 levels in living cells and can further be coupled with glucose oxidase (GOx) to achieve quantitative and selective detection of glucose in artificial urine and human serum.

引用

页码：7191 / 7197

页数：7

共 40 条

[21] Boronate-based fluorescent probes for imaging cellular hydrogen peroxide [J].

Miller, EW ;

Albers, AE ;

Pralle, A ;

Isacoff, EY ;

Chang, CJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (47) :16652-16659

[22] Unraveling the Biological Roles of Reactive Oxygen Species [J].

Murphy, Michael P. ;

Holmgren, Arne ;

Larsson, Nils-Goeran ;

Halliwell, Barry ;

Chang, Christopher J. ;

Kalyanaraman, Balaraman ;

Rhee, Sue Goo ;

Thornalley, Paul J. ;

Partridge, Linda ;

Gems, David ;

Nystrom, Thomas ;

Belousov, Vsevolod ;

Schumacker, Paul T. ;

Winterbourn, Christine C. .

CELL METABOLISM, 2011, 13 (04) :361-366

[23] Probing single molecules and single nanoparticles by surface-enhanced Raman scattering [J].

Nie, SM ;

Emery, SR .

SCIENCE, 1997, 275 (5303) :1102-1106

[24] In vivo tumor targeting and spectroscopic detection with surface-enhanced Raman nanoparticle tags [J].

Qian, Ximei ;

Peng, Xiang-Hong ;

Ansari, Dominic O. ;

Yin-Goen, Qiqin ;

Chen, Georgia Z. ;

Shin, Dong M. ;

Yang, Lily ;

Young, Andrew N. ;

Wang, May D. ;

Nie, Shuming .

NATURE BIOTECHNOLOGY, 2008, 26 (01) :83-90

[25] Size-dependent internalization of particles via the pathways of clathrin-and caveolae-mediated endocytosis [J].

Rejman, J ;

Oberle, V ;

Zuhorn, IS ;

Hoekstra, D .

BIOCHEMICAL JOURNAL, 2004, 377 :159-169

[26] H2O2, a necessary evil for cell signaling [J].

Rhee, Sue Goo .

SCIENCE, 2006, 312 (5782) :1882-1883

[27] Protein immobilization strategies for protein biochips [J].

Rusmini, Federica ;

Zhong, Zhiyuan ;

Feijen, Jan .

BIOMACROMOLECULES, 2007, 8 (06) :1775-1789

[28] Superoxide, peroxynitrite and oxidative/nitrative stress in inflammation [J].

Salvemini, D. ;

Doyle, T. M. ;

Cuzzocrea, S. .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2006, 34 :965-970

[29] Surface-Enhanced Raman Spectroscopy: Concepts and Chemical Applications [J].

Schluecker, Sebastian .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (19) :4756-4795

[30] Direct Electrochemistry of Glucose Oxidase and Biosensing for Glucose Based on Graphene [J].

Shan, Changsheng ;

Yang, Huafeng ;

Song, Jiangfeng ;

Han, Dongxue ;

Ivaska, Ari ;

Niu, Li .

ANALYTICAL CHEMISTRY, 2009, 81 (06) :2378-2382

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