Graphene oxide-chloroquine nanoconjugate induce necroptotic death in A549 cancer cells through autophagy modulation

被引：45

作者：

Arya, Braham D. ^{[1
,2
]}

Mittal, Sandeep ^{[2
,3
]}

Joshi, Prachi ^{[1
]}

Pandey, Alok K. ^{[2
,3
]}

Ramirez-Vick, Jaime E. ^{[4
]}

Singh, Surinder P. ^{[1
,2
]}

机构：

[1] CSIR Natl Phys Lab, Dr KS Krishnan Marg, New Delhi 110012, India

[2] AcSIR, Sect 19, Ghaziabad 201002, UP, India

[3] CSIR IITR, 31 Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh, India

[4] Wright State Univ, Dept Biomed Ind & Human Factors Engn, Dayton, OH 45435 USA

来源：

NANOMEDICINE | 2018年 / 13卷 / 18期

关键词：

A549; autophagosomes; autophagy; cancer therapy; chloroquine; endocytosis; graphene oxide; lysosome; nanoconjugate; necroptosis; WALLED CARBON NANOTUBES; MOLECULAR-MECHANISMS; PROGRAMMED NECROSIS; IN-VITRO; MITOCHONDRIAL DAMAGE; GRAPHITE OXIDE; DRUG-DELIVERY; APOPTOSIS; THERAPY; STRESS;

D O I：

10.2217/nnm-2018-0086

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: Chloroquine (Chl) has shown its potential in cancer therapy and graphene oxide (GO) exhibited excellent tumor-targeting ability, biocompatibility and low toxicity. We have endeavored to conjugate Chl to GO sheets and investigated the nonproliferation action on A549 cell lines along with cell signaling pathways. Materials & methods: Cellular toxicity, autophagic flux modulation and cell death mechanism induced by GO-Chl have been investigated on A549 cell lines. Results & conclusion: GO-Chl induces accumulation of autophagosomes (monodansylcadaverine staining, green fluorescence protein-tagged LC3 plasmid and transmission electron microscopy observations) in A549 cells through the blockade of autophagic flux that serves as scaffold for necrosome assembling and activates necroptotic cell death. GO-Chl nanoconjugate could be used as an effective cancer therapeutic agent, by targeting the autophagy necroptosis axis.

引用

页码：2261 / 2282

页数：22

共 76 条

[71] The role of the lateral dimension of graphene oxide in the regulation of cellular responses [J].

Yue, Hua ;

Wei, Wei ;

Yue, Zhanguo ;

Wang, Bin ;

Luo, Nana ;

Gao, Yongjun ;

Ma, Ding ;

Ma, Guanghui ;

Su, Zhiguo .

BIOMATERIALS, 2012, 33 (16) :4013-4021

[72] Interactions of graphene with mammalian cells: Molecular mechanisms and biomedical insights [J].

Zhang, Bo ;

Wei, Peng ;

Zhou, Zhixiang ;

Wei, Taotao .

ADVANCED DRUG DELIVERY REVIEWS, 2016, 105 :145-162

[73] 3D free-standing nitrogen-doped reduced graphene oxide aerogel as anode material for sodium ion batteries with enhanced sodium storage [J].

Zhang, Jiao ;

Li, Chuanqi ;

Peng, Zhikun ;

Liu, Yushan ;

Zhang, Jianmin ;

Liu, Zhongyi ;

Li, Dan .

SCIENTIFIC REPORTS, 2017, 7

[74] RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome [J].

Zhang, Yingying ;

Su, Sheng Sean ;

Zhao, Shubo ;

Yang, Zhentao ;

Zhong, Chuan-Qi ;

Chen, Xin ;

Cai, Qixu ;

Yang, Zhang-Hua ;

Huang, Deli ;

Wu, Rui ;

Han, Jiahuai .

NATURE COMMUNICATIONS, 2017, 8

[75] Graphene Oxide Induced Perturbation to Plasma Membrane and Cytoskeletal Meshwork Sensitize Cancer Cells to Chemotherapeutic Agents [J].

Zhu, Jianqiang ;

Xu, Ming ;

Gao, Ming ;

Zhang, Zhihong ;

Xu, Yong ;

Xia, Tian ;

Liu, Sijin .

ACS NANO, 2017, 11 (03) :2637-2651

[76] Molecular Mechanism of Silver Nanoparticles-Induced Human Osteoblast Cell Death: Protective Effect of Inducible Nitric Oxide Synthase Inhibitor [J].

Zielinska, Ewelina ;

Tukaj, Cecylia ;

Radomski, Marek Witold ;

Inkielewicz-Stepniak, Iwona .

PLOS ONE, 2016, 11 (10)

← 1 2 3 4 5 6 7 8 →