Graphene oxide-chloroquine nanoconjugate induce necroptotic death in A549 cancer cells through autophagy modulation

被引：45

作者：

Arya, Braham D. ^{[1
,2
]}

Mittal, Sandeep ^{[2
,3
]}

Joshi, Prachi ^{[1
]}

Pandey, Alok K. ^{[2
,3
]}

Ramirez-Vick, Jaime E. ^{[4
]}

Singh, Surinder P. ^{[1
,2
]}

机构：

[1] CSIR Natl Phys Lab, Dr KS Krishnan Marg, New Delhi 110012, India

[2] AcSIR, Sect 19, Ghaziabad 201002, UP, India

[3] CSIR IITR, 31 Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh, India

[4] Wright State Univ, Dept Biomed Ind & Human Factors Engn, Dayton, OH 45435 USA

来源：

NANOMEDICINE | 2018年 / 13卷 / 18期

关键词：

A549; autophagosomes; autophagy; cancer therapy; chloroquine; endocytosis; graphene oxide; lysosome; nanoconjugate; necroptosis; WALLED CARBON NANOTUBES; MOLECULAR-MECHANISMS; PROGRAMMED NECROSIS; IN-VITRO; MITOCHONDRIAL DAMAGE; GRAPHITE OXIDE; DRUG-DELIVERY; APOPTOSIS; THERAPY; STRESS;

D O I：

10.2217/nnm-2018-0086

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: Chloroquine (Chl) has shown its potential in cancer therapy and graphene oxide (GO) exhibited excellent tumor-targeting ability, biocompatibility and low toxicity. We have endeavored to conjugate Chl to GO sheets and investigated the nonproliferation action on A549 cell lines along with cell signaling pathways. Materials & methods: Cellular toxicity, autophagic flux modulation and cell death mechanism induced by GO-Chl have been investigated on A549 cell lines. Results & conclusion: GO-Chl induces accumulation of autophagosomes (monodansylcadaverine staining, green fluorescence protein-tagged LC3 plasmid and transmission electron microscopy observations) in A549 cells through the blockade of autophagic flux that serves as scaffold for necrosome assembling and activates necroptotic cell death. GO-Chl nanoconjugate could be used as an effective cancer therapeutic agent, by targeting the autophagy necroptosis axis.

引用

页码：2261 / 2282

页数：22

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