Two Screening Assays to Detect Vancomycin-Resistant Enterococcus spp.

被引：1

作者：

Cenci-Goga, Beniamino T. ^{[1
,2
]}

Karama, Musafiri ^{[2
]}

El-Ashram, Saeed ^{[3
,4
]}

Saraiva, Cristina ^{[5
,6
]}

Garcia-Diez, Juan ^{[5
]}

Chalias, Athanasios ^{[7
]}

Grispoldi, Luca ^{[1
]}

机构：

[1] Univ Perugia, Dipartimento Med Vet, I-06126 Perugia, Italy

[2] Univ Pretoria, Fac Vet Sci, Dept Paraclin Sci, ZA-0110 Onderstepoort, South Africa

[3] Foshan Univ, Sch Life Sci & Engn, Foshan 528231, Peoples R China

[4] Kafrelsheikh Univ, Fac Sci, Kafr Al Sheikh 33516, Egypt

[5] Univ Tras Os Montes & Alto Douro, Vet & Anim Res Ctr CECAV, P-5001801 Vila Real, Portugal

[6] Univ Tras Os Montes & Alto Douro, Sch Agr & Vet Sci, Dept Vet Sci, P-5000801 Vila Real, Portugal

[7] European Food Safety Author, EU FORA Programme, I-43126 Parma, Italy

来源：

MICROBIOLOGY RESEARCH | 2022年 / 13卷 / 02期

关键词：

enterococcus; vancomycin; antibiotic resistance; screening; MEMBRANE-FILTER TECHNIQUE; STARTER CULTURES; 2-STEP PCR; TETRACYCLINE; FAECALIS; WATER; GENE;

D O I：

10.3390/microbiolres13020026

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterococci have become major nosocomial pathogens. An increasing number of these infections are as a result of vancomycin-resistant enterococci. Accurate detection of vancomycin-resistant enterococci (VRE) is important, so that appropriate therapy and infection control measures may be instituted, including veterinary surveillance. Two screening assays to detect vancomycin resistance in enterococci are proposed. Barnes Basal Medium agar (Ba) and Brain Heart Infusion (BHI) broth (plus 1% TTC-2,3,5-triphenyltetrazolium chloride) with several concentrations of vancomycin were used in this work. Five Enterococcus casseliflavus strains with low-level resistance to vancomycin (4 mu g/mL) were used. Both media were able to quickly detect the breakpoint of the vancomycin-resistant strains used in this work, and also provided insight into the dynamics of the antibiotic effect at a low concentration on the tested bacterial suspensions.

引用

页码：332 / 341

页数：10

共 22 条

[11] Detection of Enterococcus faecalis resistant to tetracycline by a two-step PCR with nested primers [J].