Colorimetric assay for lignin peroxidase activity determination using methylene blue as substrate

被引：30
作者：
Magalhaes, DB
deCarvalho, MEA
Bon, E
Neto, JSA
Kling, SH
机构：
[1] FED UNIV RIO DE JANEIRO,ESCOLA QUIM,RIO JANEIRO,RJ,BRAZIL
[2] UNIV ESTADO RIO DE JANEIRO,INST QUIM,BR-20550000 RIO JANEIRO,RJ,BRAZIL
来源：
BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 04期
关键词：
D O I：
10.1007/BF00184028
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A colorimetric method for determination of lignin peroxidase activity has been developed which is based on the demethylation of methylene blue. The use of this dye shows the practical advantage of using a wavelength in the visible region of spectrum. The method can be efficiently used for the enzyme quantification as its sensitivity is close to the veratryl alcohol assay.
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页码：273 / 276
页数：4
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