Rapamycin Plays a Neuroprotective Effect after Spinal Cord Injury via Anti-Inflammatory Effects

被引:33
作者
Song, Yu [1 ,2 ]
Xue, Hui [1 ]
Liu, Ting-ting [3 ]
Liu, Jia-mei [1 ]
Chen, Dong [4 ]
机构
[1] Jilin Univ, Dept Histol & Embryol, Coll Basic Med Sci, Changchun 130021, Peoples R China
[2] Changchun Med Coll, Dept Anat, Changchun 130021, Peoples R China
[3] Jilin Med Coll, Affiliated Hosp, Dept Pathol, Jilin 132013, Peoples R China
[4] Guangdong Med Coll, Dept Histol & Embryol, Dongguan 523808, Peoples R China
关键词
Rapamycin; Neuron protection; Secondary damage; Rat; Spinal cord injury; Inflammation; INFLAMMATION; CANCER;
D O I
10.1002/jbt.21603
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Whether rapamycin has neuroprotective effects in spinal cord injury remains controversial. The present study shows that rapamycin protects neurons from death after spinal cord injury by inhibiting the secondary inflammatory response. The effects of rapamycin were tested using a myeloperoxidase assay, Western blotting, immunohistochemistry, and the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The experimental results showed that after spinal cord injury, rapamycin reduced the numbers of activated microglia and neutrophils in the damage zone, lowered the expression levels of TNF- and IL-1, reduced the apoptotic cells, and increased the survival of neurons. The above data proved that rapamycin diminishes inflammatory cell activation and proliferation, downregulates the expression of inflammatory factors, reduces the microenvironmental damage effects on neurons in the acute injury phase, and thus promotes the survival of neurons. Therefore, we believe that rapamycin has neuroprotective effects in spinal cord injury.
引用
收藏
页码:29 / 34
页数:6
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