Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region

被引:754
作者
Moffitt, Jeffrey R. [1 ,2 ,3 ,4 ]
Bambah-Mukku, Dhananjay [1 ,4 ,5 ]
Eichhorn, Stephen W. [1 ,2 ,3 ,4 ]
Vaughn, Eric [1 ,4 ,5 ]
Shekhar, Karthik [6 ]
Perez, Julio D. [1 ,4 ,5 ]
Rubinstein, Nimrod D. [1 ,4 ,5 ]
Hao, Junjie [1 ,2 ,3 ,4 ]
Regev, Aviv [1 ,6 ,7 ]
Dulac, Catherine [1 ,4 ,5 ]
Zhuang, Xiaowei [1 ,2 ,3 ,4 ]
机构
[1] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA
[2] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
[3] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA
[4] Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA
[5] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
[6] Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02139 USA
[7] MIT, Koch Inst Integrat Canc Biol, Dept Biol, Cambridge, MA 02139 USA
基金
美国国家卫生研究院;
关键词
GENE-EXPRESSION; STRIA TERMINALIS; BED NUCLEUS; NEURONS; CIRCUITS; AREA; SPECIFICATION; REPRODUCTION; ARCHITECTURE; BEHAVIOR;
D O I
10.1126/science.aau5324
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei-including the molecular identity, spatial organization, and function of distinct cell types-is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. We profiled similar to 1 million cells, identified similar to 70 neuronal populations characterized by distinct neuromodulatory signatures and spatial organizations, and defined specific neuronal populations activated during social behaviors in male and female mice, providing a high-resolution framework for mechanistic investigation of behavior circuits. The approach described opens a new avenue for the construction of cell atlases in diverse tissues and organisms.
引用
收藏
页码:792 / +
页数:94
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