Reversible immobilization of glucose oxidase on polyaniline grafted polyacrylonitrile conductive composite membrane

被引：48

作者：

Bayramoglu, Gulay ^{[1
]}

Metin, Ayseguel Ue ^{[2
]}

Altintas, Begum ^{[1
]}

Arica, M. Yakup ^{[1
]}

机构：

[1] Gazi Univ, Fac Arts & Sci, Biochem Proc & Biomat Res Lab, TR-06500 Ankara, Turkey

[2] Kirikkale Univ, Fac Arts & Sci, Dept Chem, TR-71450 Yahsihan, Kirikkale, Turkey

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 18期

关键词：

Conductive polymer; Polyaniline; Adsorption; Enzyme immobilization; COVALENT IMMOBILIZATION; POLYPHENOL OXIDASE; BEADS; LACCASE; LIPASE;

D O I：

10.1016/j.biortech.2010.04.025

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Polyacrylonitrile membrane surfaces were modified with chemical polymerization of conductive polyaniline. The effect of aniline concentration on the grafting efficiency was investigated As the amount of grafted PANI increased on the PAN membrane, the electrical resistance of composite membrane decreased Composite (PAN/PANI)-1-5 membrane were used for reversible immobilization of GOD. The immobilization efficiency and the activity of the immobilized GOD were increased with increasing PANI contents of the composite membrane. The maximum amount of immobilized enzyme into composite PAN/PANI-5 membrane containing 2.1% PANI was about 251 mu g/cm(2) Immobilized GOD exhibited 66% of its original activity even after two months of storage at 4 degrees C while the free enzyme lost its initial activity after three weeks (C) 2010 Elsevier Ltd. All rights reserved

引用

页码：6881 / 6887

页数：7

共 32 条

[1] Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: Application for textile dye degradation [J].

Arica, M. Yakup ;

Altintas, Begum ;

Bayramoglu, Guelay .

BIORESOURCE TECHNOLOGY, 2009, 100 (02) :665-669

[2] Invertase reversibly immobilized onto polyethylenimine-grafted poly(GMA-MMA) beads for sucrose hydrolysis [J].

Arica, MY ;

Bayramoglu, G .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2006, 38 (3-6) :131-138

[3] Polyethyleneimine-grafted poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization [J].

Arica, MY ;

Bayramoglu, G .

BIOCHEMICAL ENGINEERING JOURNAL, 2004, 20 (01) :73-77

[4]

Arica MY, 2000, POLYM INT, V49, P775

[5] Immobilization of Candida rugosa lipase onto spacer-arm attached poly(GMA-HEMA-EGDMA) microspheres [J].

Bayramoglu, G ;

Kaya, B ;

Arica, MY .

FOOD CHEMISTRY, 2005, 92 (02) :261-268

[6] Preparation of nanofibrous polymer grafted magnetic poly(GMA-MMA)-g-MAA beads for immobilization of trypsin via adsorption [J].

Bayramoglu, Guelay ;

Yilmaz, Meltem ;

Senel, Ayseguel Uelkue ;

Arica, M. Yakup .

BIOCHEMICAL ENGINEERING JOURNAL, 2008, 40 (02) :262-274

[7] Immobilization of laccase onto poly(glycidylmethacrylate) brush grafted poly(hydroxyethylmethacrylate) films: Enzymatic oxidation of phenolic compounds [J].

Bayramoglu, Guelay ;

Arica, M. Yakup .

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2009, 29 (06) :1990-1997

[8] Immobilization of β-galactosidase onto magnetic poly(GMA-MMA) beads for hydrolysis of lactose in bed reactor [J].

Bayramoglu, Gulay ;

Tunali, Yagmur ;

Arica, M. Yakup .

CATALYSIS COMMUNICATIONS, 2007, 8 (07) :1094-1101

[9] Progress in preparation, processing and applications of polyaniline [J].

Bhadra, Sambhu ;

Khastgir, Dipak ;

Singha, Nikhil K. ;

Lee, Joong Hee .

PROGRESS IN POLYMER SCIENCE, 2009, 34 (08) :783-810

[10]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

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