Line Scan Spatial Speckle Contrast Imaging and Its Application in Blood Flow Imaging

被引:5
作者
Du, E. [1 ]
Shen, Shuhao [2 ]
Qiu, Anqi [2 ]
Chen, Nanguang [2 ,3 ]
机构
[1] Shenzhen Inst Informat Technol, Sch Microelect, Shenzhen 518172, Peoples R China
[2] Natl Univ Singapore, Dept Biomed Engn, 7 Engn Dr 1, Singapore 117574, Singapore
[3] NUS Suzhou Res Inst, 377 Linquan St,Ind Pk, Suzhou 215128, Peoples R China
来源
APPLIED SCIENCES-BASEL | 2021年 / 11卷 / 22期
关键词
laser speckle; line-scan; cardiac; blood flow; confocal; chick embryo; VISUALIZATION; FLOWMETRY; VELOCITY; SKULL;
D O I
10.3390/app112210969
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Laser speckle imaging has been an indispensable tool for visualizing blood flow in biomedical applications. We proposed a novel design of the laser speckle imaging system, which combines confocal illumination and detection with various speckle analysis methods. The system can be operated by three imaging modes. One is surface illumination laser speckle contrast imaging (SI-LSCI) and the other two are line scan temporal speckle contrast imaging (LS-TSCI) and line scan spatial speckle contrast imaging (LS-SSCI). The experimental results of flow phantoms have validated the mixture model, which combines the Lorentzian and Gaussian models to describe the simultaneous existence of both Brownian motions and ordered flow. Our experimental results of in vivo chick embryos demonstrate that LS-SSCI maintains high temporal resolution and is less affected by motion artifacts. LS-SSCI can provide better image quality for in vivo imaging blood chick embryos than LS-TSCI. Furthermore, the experiential results present that LS-SSCI can detect and quantify the blood flow change during vascular clipping, and shows great potential in diagnosing vascular diseases, such as angiosclerosis, angiostenosis, or angiemphraxis.
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页数:9
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