Effect of Gold Nanorod Surface Chemistry on Cellular Response

被引:175
作者
Grabinski, Christin [2 ]
Schaeublin, Nicole [2 ]
Wijaya, Andy [3 ]
D'Couto, Helen [1 ]
Baxamusa, Salmaan H. [1 ]
Hamad-Schifferli, Kimberly [1 ,4 ]
Hussain, Saber M. [2 ]
机构
[1] MIT, Dept Biol Engn, Cambridge, MA 02139 USA
[2] USAF, Appl Biotechnol Branch, Human Effectiveness Directorate, Res Lab, Wright Patterson AFB, OH 45433 USA
[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[4] MIT, Dept Mech Engn, Cambridge, MA 02139 USA
基金
美国国家科学基金会;
关键词
nanorods; surface chemistry; cytotoxicity; gene expression; inflammation; cellular uptake; GENE-EXPRESSION; MAMMALIAN-CELLS; NANOPARTICLES; TOXICITY; CYTOTOXICITY; CANCER; SIZE; EXCHANGE; DAMAGE;
D O I
10.1021/nn103476x
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Gold nanorods (GNRs) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG(5000)) or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicological effects to HaCaT cells. GNR-PEG and GNR-MHDA exhibited minimal effects on cell proliferation, whereas GNR-CTAB reduced cell proliferation significantly due to the inherent toxicity of the cationic surfactant to cells. Cell uptake studies indicated relatively IOW uptake for GNR-PEG and high uptake for GNR-MHDA. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that GNR-PEG induced less significant and unique changes in the transcription levels of 84 genes related to stress and toxicity compared to GNR-MHDA. The results demonstrate that, although cell proliferation was not affected by both particles, there is a significant difference In gene expression in GNR-MHDA exposed cells, suggesting long-term implications for chronic exposure.
引用
收藏
页码:2870 / 2879
页数:10
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