Growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line

被引:53
作者
Alexander, HS
Huber, P
Cao, J
Krell, PJ
Nagy, E [1 ]
机构
[1] Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, ON N1G 2W1, Canada
[2] Univ Guelph, Dept Microbiol, Guelph, ON N1G 2W1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
fowl adenovirus; hepatoma cell line; growth characteristics;
D O I
10.1016/S0166-0934(98)00062-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fowl adenoviruses, many of which appear to be non-pathogenic, are ubiquitous in birds. In addition, the genome of these viruses is large, making them ideal candidates for construction as vectors for foreign genes. Current methods to cultivate fowl adenoviruses use primary cell cultures derived from embryonated chicken eggs. In order to provide a more suitable culture method, the growth of fowl adenovirus type 8 (FAdV-8) was investigated in CH-SAH, a continuous hepatoma cell line. A one step growth curve demonstrated release of extracellular virus beginning by 18 h p.i. and with a final yield about 100 fold higher than that in chicken embryo liver cells. Viral DNA synthesis was first detected 8 h prior to this. The CH-SAH cell line supported the production of progeny viruses similar to the wild-type virus after being transfected with purified FAdV-8 DNA. This study demonstrated that the continuous hepatoma cell line is an appropriate in vitro host for FAdV-8. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:9 / 14
页数:6
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