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An Enzyme Cascade Reaction for the Recovery of Hydroxytyrosol Dervatives from Olive Mill Wastewater
被引:3
作者:
Terholsen, Henrik
[1
]
Kaur, Jasmin
[1
]
Kaloudis, Nikolaos
[2
]
Staudt, Amanda
[1
]
Pavlidis, Ioannis, V
[2
]
Bornscheuer, Uwe T.
[1
]
机构:
[1] Univ Greifswald, Dept Biotechnol & Enzyme Catalysis, Felix Hausdorff Str 4, D-17487 Greifswald, Germany
[2] Univ Crete, Dept Chem, Voutes Univ Campus, Iraklion 70013, Greece
关键词:
Cascade reaction;
Flow catalysis;
Hydroxytyrosol;
Olive mill wastewaters valorization;
Promiscuous acyltransferase;
BETA-GLUCOSIDASE;
ANTIOXIDANTS;
HYDROLYSIS;
D O I:
10.1002/cite.202200069
中图分类号:
TQ [化学工业];
学科分类号:
0817 ;
摘要:
The valorization of olive mill wastewaters (OMWW), a by-product of the olive milling, is getting rising attention. Lipophilization of the main phenolic compound 3-hydroxytyrosol (HT) could facilitate its extraction. An immobilized variant of the promiscuous hydrolase/acyltransferase from Pyrobaculum calidifontis VA1 (PestE) was used to perform acetylation in water using ethyl acetate as acyl donor. PestE was used in a segmented flow setting to allow continuous operation. Additionally, HT precursors were made accessible by pretreatment with almond beta-glucosidase and the hydrolytic activity of PestE_I208A_L209F_N288A.
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页码:1860 / 1863
页数:4
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