An Enzyme Cascade Reaction for the Recovery of Hydroxytyrosol Dervatives from Olive Mill Wastewater

被引:3
|
作者
Terholsen, Henrik [1 ]
Kaur, Jasmin [1 ]
Kaloudis, Nikolaos [2 ]
Staudt, Amanda [1 ]
Pavlidis, Ioannis, V [2 ]
Bornscheuer, Uwe T. [1 ]
机构
[1] Univ Greifswald, Dept Biotechnol & Enzyme Catalysis, Felix Hausdorff Str 4, D-17487 Greifswald, Germany
[2] Univ Crete, Dept Chem, Voutes Univ Campus, Iraklion 70013, Greece
关键词
Cascade reaction; Flow catalysis; Hydroxytyrosol; Olive mill wastewaters valorization; Promiscuous acyltransferase; BETA-GLUCOSIDASE; ANTIOXIDANTS; HYDROLYSIS;
D O I
10.1002/cite.202200069
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The valorization of olive mill wastewaters (OMWW), a by-product of the olive milling, is getting rising attention. Lipophilization of the main phenolic compound 3-hydroxytyrosol (HT) could facilitate its extraction. An immobilized variant of the promiscuous hydrolase/acyltransferase from Pyrobaculum calidifontis VA1 (PestE) was used to perform acetylation in water using ethyl acetate as acyl donor. PestE was used in a segmented flow setting to allow continuous operation. Additionally, HT precursors were made accessible by pretreatment with almond beta-glucosidase and the hydrolytic activity of PestE_I208A_L209F_N288A.
引用
收藏
页码:1860 / 1863
页数:4
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