Dynamic and non-contact 3D sample rotation for microscopy

被引：22

作者：

Berndt, Frederic ^{[1
,2
,3
]}

Shah, Gopi ^{[1
,4
]}

Power, Rory M. ^{[1
,5
]}

Brugues, Jan ^{[1
,2
,3
]}

Huisken, Jan ^{[1
,5
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany

[2] Max Planck Inst Phys Komplexer Syst, Nothnitzer Str 38, D-01187 Dresden, Germany

[3] Ctr Syst Biol Dresden, Pfotenhauerstr 108, D-01307 Dresden, Germany

[4] European Mol Biol Lab, Carrer Dr Aiguader 88, Barcelona 08003, Spain

[5] Morgridge Inst Res, 330 N Orchard St, Madison, WI 53715 USA

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

欧洲研究理事会;

关键词：

D O I：

10.1038/s41467-018-07504-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.

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页数：7

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