Impacts of bio-stimulants on pyrene degradation, prokaryotic community compositions, and functions

被引：29

作者：

Ahmad, Manzoor ^{[1
]}

Wang, Pandeng ^{[1
]}

Li, Jia-Ling ^{[1
]}

Wang, Renfei ^{[3
]}

Duan, Li ^{[1
]}

Luo, Xiaoqing ^{[1
]}

Irfan, Muhammad ^{[4
]}

Peng, Ziqi ^{[1
]}

Yin, Lingzi ^{[1
]}

Li, Wen-Jun ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Guangdong Prov Key Lab, Plant Resources & Southern Marine Sci & Engn Guan, Sch Life Sci,State Key Lab Biocontrol, Guangzhou 510275, Peoples R China

[2] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China

[3] UCL, Div Biosci, Gower St, London WC1E 6BT, England

[4] Univ Florida, Dept Oral Biol, Coll Dent, Gainesville, FL USA

来源：

ENVIRONMENTAL POLLUTION | 2021年 / 289卷

基金：

中国国家自然科学基金;

关键词：

Pearl river sediment; Bio-stimulation; Bacterial community succession; 16S rDNA amplicon sequencing; qPCR; PAH-RHD genes; POLYCYCLIC AROMATIC-HYDROCARBONS; MICROBIAL COMMUNITY; BACTERIAL DIVERSITY; SOIL; SEDIMENT; PAHS; BIOCHAR; PHENANTHRENE; RESPONSES; GENES;

D O I：

10.1016/j.envpol.2021.117863

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Bio-stimulation of the indigenous microbial community is considered as an effective strategy for the bioreme-diation of polluted environments. This examination explored the near effects of various bio-stimulants on pyrene degradation, prokaryotic community compositions, and functions using 16S rRNA amplicon sequencing and qPCR. At first, the results displayed significant differences (p < 0.05) between the prokaryotic community structures of the control group, PYR (contains pyrene only), and bio-stimulants amended groups. Among the bio-stimulants, biochar, oxalic acid, salicylate, NPK, and ammonium sulfate augmented the pyrene degradation potential of microbial communities. Moreover, the higher abundance of genera, such as Flavobacterium, Hydrogenophaga, Mycobacterium, Rhodococcus, Flavihumibacter, Pseudomonas, Novosphingobium, etc., across the treatments indicated that these genera play a vital role in pyrene metabolism. Based on the higher abundance of GP-RHD and nidA genes, we speculated that Gram-positive prokaryotic communities are more competent in pyrene dissipation than Gram-negative. Furthermore, the marked abundance of nifH, and pqqC genes in the NPK and SA treatments, respectively, suggested that different bio-stimulants might enrich certain bacterial assem-blages. Besides, the significant distinctions (p < 0.05) between the bacterial consortia of HA (humic acid) and SA (sodium acetate) groups from NPK, OX (oxalic acid), UR (urea), NH4, and SC (salicylate) groups also suggested that different bio-stimulants might induce distinct ecological impacts influencing the succession of prokaryotic communities in distinct directions. This work provides new insight into the bacterial degradation of pyrene using the bio-stimulation technique. It suggests that it is equally important to investigate the community structure and functions along with studying their impacts on degradation when devising a bio-stimulation technology.

引用

页数：11

共 56 条

[1] The distinct response of phenanthrene enriched bacterial consortia to different PAHs and their degradation potential: a mangrove sediment microcosm study [J].