Expression of inhibitor of apoptosis protein Livin in renal cell carcinoma and non-tumorous adult kidney

被引:30
作者
Wagener, N.
Crnkovic-Mertens, I.
Vetter, C.
Macher-Goeppinger, S.
Bedke, J.
Groene, E. F.
Zentgraf, H.
Pritsch, M.
Hoppe-Seyler, K.
Buse, S.
Haferkamp, A.
Autschbach, F.
Hohenfellner, M.
Hoppe-Seyler, F.
机构
[1] German Canc Res Ctr, D-69120 Heidelberg, Germany
[2] Heidelberg Univ, Dept Urol, D-69120 Heidelberg, Germany
[3] Heidelberg Univ, Dept Pathol, D-69120 Heidelberg, Germany
[4] Heidelberg Univ, Dept Med Biometry, D-69120 Heidelberg, Germany
关键词
inhibitor of apoptosis; Livin/ML-IAP/KIAP; renal cell carcinoma; tumour therapy;
D O I
10.1038/sj.bjc.6604028
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The antiapoptotic Livin/ML-IAP gene has recently gained much attention as a potential new target for cancer therapy. Reports indicating that livin is expressed almost exclusively in tumours, but not in the corresponding normal tissue, suggested that the targeted inhibition of livin may present a novel tumour-specific therapeutic strategy. Here, we compared the expression of livin in renal cell carcinoma and in non-tumorous adult kidney tissue by quantitative real-time reverse transcription-PCR, immunoblotting, and immunohistochemistry. We found that livin expression was significantly increased in tumours (P=0.0077), but was also clearly detectable in non-tumorous adult kidney. Transcripts encoding Livin isoforms alpha and beta were found in both renal cell carcinoma and normal tissue, without obvious qualitative differences. Livin protein in renal cell carcinoma samples exhibited cytoplasmic and/or nuclear staining. In non-tumorous kidney tissue, Livin protein expression was only detectable in specific cell types and restricted to the cytoplasm. Thus, whereas the relative overexpression of livin in renal cell carcinoma indicates that it may still represent a therapeutic target to increase the apoptotic sensitivity of kidney cancer cells, this strategy is likely to be not tumour-specific.
引用
收藏
页码:1271 / 1276
页数:6
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