Enzymatic rearrangement of chitine hydrolysates with β-N-acetylhexosaminidase from Aspergillus oryzae

被引：14
|
作者：
Dvoráková, J
Schmidt, D
Hunková, Z
Thiem, J
Kren, V
机构：
[1] Acad Sci Czech Republ, Inst Microbiol, Lab Biotransformat, CZ-14220 Prague 4, Czech Republic
[2] Univ Hamburg, Inst Inorgan Chem, D-20146 Hamburg, Germany
来源：
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2001年 / 11卷 / 4-6期
关键词：
chitooligosaccharides; beta-N-acetylhexosaminidase; Aspergillus oryzae; enzymatic transglycosylation;
D O I：
10.1016/S1381-1177(00)00075-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The role of higher chitooligomers in medical applications is increasing due to their interesting biological activities. Transglycosylation activity of beta -N-acetylhexosaminidase from Aspergillus oryzae was employed to produce higher chitooligosaccharides (chitohexaose-chitooctaose) from a mixture of lower chitooligomers prepared by acid hydrolysis of chitin. Enzymatic rearrangement of the chitooligomer mixture was optimized in respect of substrate concentration, presence of inorganic salts, enzyme activity, and reaction time to achieve the highest production of longer chitooligomers. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：225 / 232
页数：8
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