Effects of Anodized Titanium Implant Coated with RGD Peptides via Chemical Fixation on Osseointegration and Bone Regeneration

This study was conducted to investigate the effects of an anodized titanium surface with RGD-peptides. Silane was used to immobilize the RGD peptides to the titanium surfaces. The surfaces were examined by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Crystal violet assay, XTT assay and Alkaline phosphatase (ALP) assay were performed to evaluate response of human mesenchymal stem cell. For in vivo study, twenty-four implants and four beagle dogs were used and made the dehiscence defect model (n=12 in each group). Implant stability quotient (ISQ) value was measured and evaluated by histometric analysis. Histological examination revealed an increase of bone height in the test group. There were significant differences in intra-thread bone density (ITBD) between the groups. The application of RGD peptide to the implant surface may contribute to the improvement of osseointegration quality and bone growth in a small defect area around the implant.