Analysis of complex formation between crown ethers and potassium ion by determining retention factors in reversed-phase high-performance liquid chromatography

Retention factors in reversed-phase high-performance liquid chromatography (RP-HPLC) were utilized for the analysis of complex formation reaction of four crown ethers with potassium ion in water-methanol media. The crown ethers were injected as analytes, potassium ion was present in the eluent, and three types of reversed-phase columns were used. The retention factor of the crown ethers decreased with increasing concentrations of potassium ion in the eluent, which is attributed to the change in the species of the crown ether from a neutral ligand to a positively charged complex. The complex formation constants were determined by analyzing the changes in the retention factor with a non-linear least-squares method. (C) 2001 Elsevier Science B.V. All rights reserved.