In vitro growth, cytopathic effects and clearance of monolayers by clinical isolates of Balamuthia mandrillaris in human skin cell cultures

被引：5

作者：

Yera, Helene ^{[1
,2
,3
]}

Dupouy-Camet, Jean ^{[3
]}

Jackson, Jonathan W. ^{[1
,2
]}

Sriram, Rama ^{[1
,2
]}

Sweat, Stacey ^{[4
]}

Goldstein, Jason M. ^{[4
]}

Visvesvara, Govinda S. ^{[1
,2
]}

机构：

[1] Ctr Dis Control & Prevent, Lab Free Living Amebae, Div Foodborne Waterborne & Environm Dis, Atlanta, GA 30333 USA

[2] Ctr Dis Control & Prevent, Div Sci Resources, Natl Ctr Environm & Zoonot Infect Dis, Atlanta, GA 30333 USA

[3] Univ Paris 05, Lab Parasitol Mycol, Hop Cochin, AP HP,Hop Univ Paris Ctr,Fac Med, F-75014 Paris, France

[4] Ctr Dis Control & Prevent, Sci Prod & Support Branch, Div Sci Resources, Natl Ctr Environm & Zoonot Infect Dis, Atlanta, GA 30333 USA

来源：

EXPERIMENTAL PARASITOLOGY | 2015年 / 156卷

关键词：

Balamuthia mandrillaris; Human skin; Cell culture; Cytopathic effect; FREE-LIVING AMEBAS; ACANTHAMOEBA-KERATITIS; AGENT; ENCEPHALITIS; MENINGOENCEPHALITIS; CYTOPATHOGENICITY; ANTIBODIES; ANIMALS; SAMPLES;

D O I：

10.1016/j.exppara.2015.05.004

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Balamuthia mandrillaris is a free-living ameba (FLA) that has been isolated or its DNA identified in soil, dust and water. It causes a fatal central nervous system infection in humans and animals. Although it is environmental as Acanthamoeba and Naegleria fowleri, the two other free-living amebae that also cause CNS infections in humans and other animals, Balamuthia does not feed on bacteria as the other FLA. In the laboratory, it can be grown on a variety of mammalian cell cultures. In this study we examined the ability of three different Balamuthia isolates to grow on several different human skin cell cultures including the WT/A keratinocyte cell cultures. A corneal isolate of Acanthamoeba castellanii was used for comparison. (C) 2015 Published by Elsevier Inc.

引用

页码：61 / 67

页数：7

共 29 条

[1] Development of a Nested PCR for Environmental Detection of the Pathogenic Free-Living Amoeba Balamuthia mandrillaris [J].