Determination of Macrolide Antibiotics Using Dispersive Liquid-Liquid Microextraction Followed by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

被引：18

作者：

Chen, Kuan-Yu ^{[1
]}

Yang, Thomas C. ^{[1
]}

Chang, Sarah Y. ^{[1
]}

机构：

[1] Natl Kaohsiung Normal Univ, Dept Chem, Kaohsiung 824, Taiwan

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2012年 / 23卷 / 06期

关键词：

Dispersive liquid-liquid microextraction; Macrolide antibiotics; SALDI/MS; Urine sample; SILVER NANOPARTICLES; HUMAN URINE; CHROMATOGRAPHY; EXTRACTION; RESIDUES; MATRICES;

D O I：

10.1007/s13361-012-0371-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method for the determination of macrolide antibiotics using dispersive liquid-liquid microextraction coupled to surface-assisted laser desorption/ionization mass spectrometric detection was developed. Acetone and dichloromethane were used as the disperser solvent and extraction solvent, respectively. A mixture of extraction solvent and disperser solvent were rapidly injected into a 1.0 mL aqueous sample to form a cloudy solution. After the extraction, macrolide antibiotics were detected using surface-assisted laser desorption/ionization mass spectrometry (SALDI/MS) with colloidal silver as the matrix. Under optimum conditions, the limits of detection (LODs) at a signal-to-noise ratio of 3 were 2, 3, 3, and 2 nM for erythromycin (ERY), spiramycin (SPI), tilmicosin (TILM), and tylosin (TYL), respectively. This developed method was successfully applied to the determination of macrolide antibiotics in human urine samples.

引用

页码：1157 / 1160

页数：4

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