Chromatographic characterization of proanthocyanidins after thiolysis with cysteamine

被引：26

作者：

Torres, JL ^{[1
]}

Lozano, C ^{[1
]}

机构：

[1] CSIC, IIQAB, Inst Chem & Environm Res, Dept Peptide & Prot Chem, Barcelona 08034, Spain

来源：

CHROMATOGRAPHIA | 2001年 / 54卷 / 7-8期

关键词：

column liquid chromatography; proanthocyanidins; thiolysis with cysteamine; flavan-3-ols; polyphenols;

D O I：

10.1007/BF02491211

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cysteamine is proposed as a user-friendly thiol donor with application to the analysis of proanthocyanidins by thiolysis. Oligomeric proanthocyanidins are potent antioxidants and disease-preventing agents. The efficiency of which depends on their composition and size. The degree of polymerization of proanthocyanidins is usually estimated by thiolysis then reversed-phase high-performance liquid chromatography. The new derivatization procedure is an alternative to the use of toluene-alpha -thiol as thiol donor. In addition to enabling the direct chromatographic analysis of crude material, the amino function introduced facilitates prior discrimination between terminal and extension flavanoid moieties by means of cation-exchange chromatography.

引用

页码：523 / 526

页数：4

共 17 条

[1] Comparative study of polyphenol scavenging activities assessed by different methods [J].

de Gaulejac, NS ;

Provost, C ;

Vivas, N .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (02) :425-431

[2] Functional food science and defence against reactive oxidative species [J].

Diplock, AT ;

Charleux, JL ;

Crozier-Willi, G ;

Kok, FJ ;

Rice-Evans, C ;

Roberfroid, M ;

Stahl, W ;

Viña-Ribes, J .

BRITISH JOURNAL OF NUTRITION, 1998, 80 :S77-S112

[3] Reversed-phase HPLC following thiolysis for quantitative estimation and characterization of the four main classes of phenolic compounds in different tissue zones of a French cider apple variety (Malus domestica var. Kermerrien) [J].

Guyot, S ;

Marnet, N ;

Laraba, D ;

Sanoner, P ;

Drilleau, JF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (05) :1698-1705

[4] Method for estimation of proanthocyanidins based on their acid depolymerization in the presence of nucleophiles [J].

Matthews, S ;

Mila, I ;

Scalbert, A ;

Pollet, B ;

Lapierre, C ;

duPenhoat, CLMH ;

Rolando, C ;

Donnelly, DMX .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (04) :1195-1201

[5] Antioxidant properties of catechins and proanthocyanidins: Effect of polymerisation, galloylation and glycosylation [J].

Plumb, GW ;

De Pascual-Teresa, S ;

Santos-Buelga, C ;

Cheynier, V ;

Williamson, G .

FREE RADICAL RESEARCH, 1998, 29 (04) :351-358

[6] PHYLOGENETIC CONSTRAINTS, ADAPTIVE SYNDROMES, AND EMERGENT PROPERTIES - FROM INDIVIDUALS TO POPULATION-DYNAMICS [J].

PRICE, PW .

RESEARCHES ON POPULATION ECOLOGY, 1994, 36 (01) :3-14

[7] MICRO METHOD FOR THE IDENTIFICATION OF PROANTHOCYANIDIN USING THIOLYSIS MONITORED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

RIGAUD, J ;

PEREZILZARBE, J ;

RICARDO-DA-SILVA, JM ;

CHEYNIER, V .

JOURNAL OF CHROMATOGRAPHY, 1991, 540 (1-2) :401-405

[8]

Ruf JC, 1999, DRUG EXP CLIN RES, V25, P125

[9] Polyphenol profiles of French cider apple varieties (Malus domestica sp.) [J].

Sanoner, P ;

Guyot, S ;

Marnet, N ;

Molle, D ;

Drilleau, JF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (12) :4847-4853

[10]

Soleas GJ, 1997, J CLIN LAB ANAL, V11, P287, DOI 10.1002/(SICI)1098-2825(1997)11:5<287::AID-JCLA6>3.0.CO

← 1 2 →