Characterization and SSR identify of the complete chloroplast genome of Paphiopedilum concolor (Orchidaceae)

被引:2
|
作者
Hua, Wenping [1 ]
Chen, Chen [2 ,3 ]
机构
[1] Shaanxi XueQian Normal Univ, Dept Life Sci & Food Technol, Changan, Peoples R China
[2] Inst Bot Shaanxi Prov, Xian Bot Garden Shaanxi Prov, Cuihua South, Peoples R China
[3] Shaanxi Engn Res Ctr Conservat & Utilizat Bot Res, Cuihua South, Peoples R China
来源
MITOCHONDRIAL DNA PART B-RESOURCES | 2019年 / 4卷 / 01期
基金
中国国家自然科学基金;
关键词
Paphiopedilum concolor; chloroplast genome; microsatellites; MITObim;
D O I
10.1080/23802359.2019.1586463
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Paphiopedilum concolor (Orchidaceae) is endemic to China with highly ornamental and horticultural value. However, its whole chloroplast (cp) genome has not been reported yet. Here, the first nucleotide sequence of P. concolor was identified using Illumina sequencing technology. The circular genome is 166,744 bp in size and comprises a pair of inverted repeat (IR) regions of 15,694 bp, a large single-copy (LSC) region of 139,351 bp, and a small single-copy (SSC) region of 11,729 bp. The total GC content is 34.6%, whereas the corresponding values of the LSC, SSC, and IR regions are 33.7%, 26.0%, and 41.6%, respectively. The chloroplast genome contains 137 genes, including 91 protein-coding genes, eight ribosomal RNA genes, and 38 transfer RNA genes. Moreover, we identified a total of 129 microsatellites (SSRs) within the P. concolor cp genome. These newly developed genomic resources will greatly important to the further conservation genetic studies of Paphiopedilum species.
引用
收藏
页码:1074 / 1076
页数:3
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