Functional nanozyme mediated multi-readout and label-free lateral flow immunoassay for rapid detection of Escherichia coli O157:H7

被引：94

作者：

Wang, Zonghan ^{[1
]}

Yao, Xiaolin ^{[1
]}

Zhang, Yongzhi ^{[1
]}

Wang, Rong ^{[1
]}

Ji, Yanwei ^{[1
]}

Sun, Jing ^{[2
]}

Zhang, Daohong ^{[1
]}

Wang, Jianlong ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Food Sci & Engn, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China

[2] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810008, Qinghai, Peoples R China

来源：

FOOD CHEMISTRY | 2020年 / 329卷

基金：

中国国家自然科学基金;

关键词：

Label-free; Multi-readout; Nanozyme; Lateral flow immunoassay; Mannose modified Prussian blue; Escherichia coli O157:H7; CARBON NANOPARTICLES; SENSITIVE DETECTION; GRAPHENE OXIDE; ASSAY; STRIP; GOLD; ADSORPTION; BIOSENSOR;

D O I：

10.1016/j.foodchem.2020.127224

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To overcome the drawbacks of antibody labeling dependence and single-readout system in the conventional lateral flow immunoassays (LFIAs) as well as the non-targeted combination of new capture agents reported recently for pathogen detection, in this work, a multi-readout and label-free LFIA was proposed for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) based on a nanozyme-bacteria-antibody sandwich pattern. A type of functional nanozyme-mannose modified Prussian blue (man-PB), was introduced as the recognition agent as well as signal indicator. Apart from original signal intensity on the T-line, the peroxidase-like catalytic activity-driven generation of colorimetric signal could be used as another format of quantitation. Importantly, such LFIA could exhibit excellent performance for target pathogens detection separately with a quantitative range of 10(2)-10(8) cfu.mL(-1) and a low detection limit of 10(2) cfu.mL(-1) based on different readout formats, indicating the application potential of the proposed LFIA in real samples.

引用

页数：7

共 38 条

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