Anxiety-like behaviors and expression of SERT and TPH in the dorsal raphe of estrogen- and fluoxetine-treated ovariectomized rats

被引:39
作者
Charoenphandhu, Jantarima [1 ]
Teerapornpuntakit, Jarinthorn [2 ]
Nuntapornsak, Amporn [2 ]
Krishnamra, Nateetip [2 ]
Charoenphandhu, Narattaphol [2 ]
机构
[1] Thammasat Univ, Fac Med, Div Physiol, Pathum Thani 12120, Thailand
[2] Mahidol Univ, Fac Sci, Dept Physiol, Bangkok 10400, Thailand
关键词
Anxiety; Elevated plus-maze; Elevated T-maze; Open field; Real-time PCR; Selective serotonin reuptake inhibitor (SSRI); Tryptophan hydroxylase (TPH); SEROTONIN REUPTAKE INHIBITORS; ELEVATED PLUS-MAZE; MESSENGER-RNA; POSTMENOPAUSAL WOMEN; GENE-EXPRESSION; PSYCHIATRIC-DISORDERS; MONOAMINE-OXIDASE; 5-HT1A RECEPTORS; OPEN-FIELD; T-MAZE;
D O I
10.1016/j.pbb.2011.02.023
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
The anxiolytic effect of fluoxetine (Flx) was often ineffective in postmenopausal and estrogen-deficient patients, but such effect had not been experimentally demonstrated, particularly in the female rat model of estrogen deficiency. Here we determined the anxiety-like behaviors in ovariectomized (Ovx) rats treated for 4 weeks with 10 mu g/kg 17 beta-estradiol s.c. (Ovx + E2), 10 mg/kg Flx p.o. (Ovx + Flx) or a combination of both (Ovx + E2 + Flx). Since Fix is known to induce anxiolysis in males, we first evaluated the Flx regimen in male rats. The results showed that anxiety-like behaviors were reduced in Flx-treated male rats. In contrast, Ovx + Flx rats still exhibited the same anxiety-like behaviors as in Ovx rats. Both Ovx + E2 and Ovx + E2 + Flx rats, however, showed comparable reductions in anxiety-like behaviors, suggesting that Flx had no anxiolytic-like effect. Furthermore, E2 and E2 + Fix similarly upregulated the mRNA expression of serotonin reuptake transporter (SERT) and tryptophan hydroxylase-2 in the dorsal raphe of Ovx rats, while having no effect on SERT expression in the frontal cortex, hippocampus, septum, amygdala and periaqueductal gray. In conclusion, Flx induced anxiolytic-like action in male rats. In Ovx rats, it was E2 and not Flx that exerted the anxiolytic-like action, which was mediated, in part, by altering serotonin metabolism in the dorsal raphe. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:503 / 510
页数:8
相关论文
共 58 条
[1]   SERUM ESTRADIOL CONCENTRATION REQUIRED TO MAINTAIN BODY-WEIGHT, ATTRACTIVITY, PROCEPTIVITY, AND RECEPTIVITY IN THE OVARIECTOMIZED FEMALE RAT [J].
ALBERT, DJ ;
JONIK, RH ;
GORZALKA, BB ;
NEWLOVE, T ;
WEBB, B ;
WALSH, ML .
PHYSIOLOGY & BEHAVIOR, 1991, 49 (02) :225-231
[2]  
Amin Zenab, 2005, Behav Cogn Neurosci Rev, V4, P43, DOI 10.1177/1534582305277152
[3]   Antidepressants Targeting the Serotonin Reuptake Transporter Act via a Competitive Mechanism [J].
Apparsundaram, Subbu ;
Stockdale, Daniel J. ;
Henningsen, Robert A. ;
Milla, Marcos E. ;
Martin, Renee S. .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2008, 327 (03) :982-990
[4]  
Arpels JC, 1996, J REPROD MED, V41, P633
[5]   Diverse actions of ovarian steroids in the serotonin neural system [J].
Bethea, CL ;
Lu, NZ ;
Gundlah, C ;
Streicher, JM .
FRONTIERS IN NEUROENDOCRINOLOGY, 2002, 23 (01) :41-100
[6]   Effects of oral estrogen, raloxifene and arzoxifene on gene expression in serotonin neurons of macaques [J].
Bethea, CL ;
Mirkes, SJ ;
Su, A ;
Michelson, D .
PSYCHONEUROENDOCRINOLOGY, 2002, 27 (04) :431-445
[7]   Psychologic distress and natural menopause: A multiethnic community study [J].
Bromberger, JT ;
Meyer, PM ;
Kravitz, HM ;
Sommer, B ;
Cordal, A ;
Powell, L ;
Ganz, PA ;
Sutton-Tyrrell, K .
AMERICAN JOURNAL OF PUBLIC HEALTH, 2001, 91 (09) :1435-1442
[8]   Nongenomic steroidal modulation of high-affinity serotonin transport [J].
Chang, AS ;
Chang, SM .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1417 (01) :157-166
[9]   Two-step stimulation of intestinal Ca2+ absorption during lactation by long-term prolactin exposure and suckling-induced prolactin surge [J].
Charoenphandhu, Narattaphol ;
Nakkrasae, La-iad ;
Kraidith, Kamonshanok ;
Teerapornpuntakit, Jarinthorn ;
Thongchote, Kanogwun ;
Thongon, Narongrit ;
Krishnamra, Nateetip .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2009, 297 (03) :E609-E619
[10]   Psychiatric Disorders and Cognitive Dysfunction Among Older, Postmenopausal Women: Results From the Women's Health Initiative Memory Study [J].
Colenda, Christopher C. ;
Legault, Claudine ;
Rapp, Stephen R. ;
DeBon, Margaret W. ;
Hogan, Patricia ;
Wallace, Robert ;
Hershey, Linda ;
Ockene, Judith ;
Whitmer, Rachael ;
Phillips, Lawrence S. ;
Sarto, Gloria E. .
AMERICAN JOURNAL OF GERIATRIC PSYCHIATRY, 2010, 18 (02) :177-185