Biophysical measurements of cells, microtubules, and DNA with an atomic force microscope

被引：6

作者：

Devenica, Luka M. ^{[1
]}

Contee, Clay ^{[1
]}

Cabrejo, Raysa ^{[1
]}

Kurek, Matthew ^{[1
]}

Deveney, Edward F. ^{[2
]}

Carter, Ashley R. ^{[1
]}

机构：

[1] Amherst Coll, Dept Phys, Amherst, MA 01002 USA

[2] Bridgewater State Univ, Dept Phys, Bridgewater, MA 02325 USA

来源：

AMERICAN JOURNAL OF PHYSICS | 2016年 / 84卷 / 04期

基金：

美国国家科学基金会;

关键词：

AFM; REGISTRATION; STABILITY; MOLECULES; FILAMENTS;

D O I：

10.1119/1.4941048

中图分类号：

G40 [教育学];

学科分类号：

040101 ; 120403 ;

摘要：

Atomic force microscopes (AFMs) are ubiquitous in research laboratories and have recently been priced for use in teaching laboratories. Here, we review several AFM platforms and describe various biophysical experiments that could be done in the teaching laboratory using these instruments. In particular, we focus on experiments that image biological materials (cells, microtubules, and DNA) and quantify biophysical parameters including membrane tension, persistence length, contour length, and the drag force. (C) 2016 American Association of Physics Teachers.

引用

页码：301 / 310

页数：10

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