Impurity analysis of gentamicin bulk samples by improved liquid chromatography-ion trap mass spectrometry

被引：2
作者：
Zheng Chao [1 ,2 ]
Wang BoChu [1 ]
Wang MingJuan [2 ,3 ]
Hoogmartens Jos [2 ]
Schepdael Ann Van [2 ]
Adams Erwin [2 ]
机构：
[1] Chongqing Univ, Bioengn Coll, Chongqing 400030, Peoples R China
[2] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Anal, B-3000 Louvain, Belgium
[3] Natl Inst Control Pharmaceut & Biol Prod, Beijing 100050, Peoples R China
来源：
SCIENCE CHINA-CHEMISTRY | 2011年 / 54卷 / 09期
关键词：
gentamicin; liquid chromatography; LC/MS; impurity analysis; PULSED ELECTROCHEMICAL DETECTION; AMINOGLYCOSIDE ANTIBIOTICS; AMINOGLYOSIDE ANTIBIOTICS; CAPILLARY DERIVATIZATION; COLUMN DERIVATIZATION; ULTRAVIOLET DETECTION; MICRO-DETERMINATION; COMPONENT RATIO; SULFATE; SEPARATION;
D O I：
10.1007/s11426-011-4304-0
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Several gentamicin bulk samples from different origins were investigated using an LC/MS method. LC equipped with ion trap MS with positive ionization was performed on a Capcell Pak C(18) (AQ) column with the mobile phase containing 50 mM trifluoroacetic (TFA) and methanol. Impurities present in batches of gentamicin bulk samples were elucidated and compared according to their fragmentation behavior. In total seventeen impurities present in samples, five impurities were not elucidated and two compounds were identified preliminarily. It was observed that the impurity profiles were different in samples from different origins which indicate necessity in the quality control of gentamicin.
引用
收藏
页码：1518 / 1528
页数：11
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