Application of continuous supercritical anti-solvents for rapid recrystallization and purification of zeaxanthin dipalmitates from de-glycosides of Lycium barbarum fruits

被引:15
作者
Chang, Li-Pin [1 ]
Cheng, Jia-Hui [1 ]
Hsu, Shih-Lan [3 ]
Liau, Bing-Chung [1 ]
Wu, Tzong-Ming [2 ]
Chang, Chieh-Ming J. [1 ]
机构
[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan
[2] Natl Chung Hsing Univ, Dept Mat Sci & Engn, Taichung 402, Taiwan
[3] Taichung Vet Gen Hosp, Educ & Res Dept, Taichung 407, Taiwan
关键词
Lycium barbarum; Zeaxanthin palmitates; Supercritical anti-solvent recrystallization; Anti-oxidative ability; Human adult retinal pigment epithelial cells; MACULAR PIGMENT; CAROTENOIDS; EXTRACTION; LYCOPENE;
D O I
10.1016/j.supflu.2011.02.008
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
This study employed column elution fractionation coupled with supercritical anti-solvent recrystallization to isolate the purest zeaxanthin dipalmitates (ZP), one of the eye-protecting compounds in the Lycium barbarum fruits. The amount of ZP increased to 983 mg/gin liquid anti-solvent precipitates with 29.1% recovery and increased to 929 mg/gin supercritical anti-solvent precipitates with 59.6% recovery, respectively from the ultrasonic acetone extract. Experimental data showed that supercritical anti-solvent recrystallization is feasible in producing platelet type precipitates within a few minutes. These ZP compounds exhibit anti-oxidative abilities by proliferating human adult retinal pigment epithelial cells (from 22% to 31%); though it was a meager increase when compared to those of algal 95% zeaxanthin (from 43% to 94%). (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:155 / 161
页数:7
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