Application of continuous supercritical anti-solvents for rapid recrystallization and purification of zeaxanthin dipalmitates from de-glycosides of Lycium barbarum fruits
This study employed column elution fractionation coupled with supercritical anti-solvent recrystallization to isolate the purest zeaxanthin dipalmitates (ZP), one of the eye-protecting compounds in the Lycium barbarum fruits. The amount of ZP increased to 983 mg/gin liquid anti-solvent precipitates with 29.1% recovery and increased to 929 mg/gin supercritical anti-solvent precipitates with 59.6% recovery, respectively from the ultrasonic acetone extract. Experimental data showed that supercritical anti-solvent recrystallization is feasible in producing platelet type precipitates within a few minutes. These ZP compounds exhibit anti-oxidative abilities by proliferating human adult retinal pigment epithelial cells (from 22% to 31%); though it was a meager increase when compared to those of algal 95% zeaxanthin (from 43% to 94%). (C) 2011 Elsevier B.V. All rights reserved.
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Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, EnglandManchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Beatty, S
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Boulton, M
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机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Boulton, M
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Henson, D
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机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Henson, D
;
Koh, HH
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机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Koh, HH
;
Murray, IJ
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机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
机构:
Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China
Cheng, CY
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Chung, WY
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Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China
Chung, WY
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Szeto, YT
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Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China
Szeto, YT
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Benzie, IFF
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Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China
机构:
Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, EnglandManchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Beatty, S
;
Boulton, M
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h-index: 0
机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Boulton, M
;
Henson, D
论文数: 0引用数: 0
h-index: 0
机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Henson, D
;
Koh, HH
论文数: 0引用数: 0
h-index: 0
机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
Koh, HH
;
Murray, IJ
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h-index: 0
机构:Manchester Royal Eye Hosp, Univ Dept Ophthalmol, Manchester, Lancs, England
机构:
Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China
Cheng, CY
;
Chung, WY
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Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China
Chung, WY
;
Szeto, YT
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h-index: 0
机构:
Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China
Szeto, YT
;
Benzie, IFF
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Hong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Fac Hlth & Social Sci, Antioxidant Res Grp, Kowloon, Hong Kong, Peoples R China