Effects of 4-Hydroxy-2-Nonenal, a Major Lipid Peroxidation-Derived Aldehyde, and N-Acetylcysteine on the Cyclooxygenase-2 Expression in Human Uterine Myometrium

被引:6
作者
Temma-Asano, Kumiko [2 ]
Tskitishvili, Ekaterine [2 ]
Kanagawa, Takeshi [2 ]
Tomimatsu, Takuji [2 ]
Tsutsui, Tateki [2 ]
Kimura, Tadashi [2 ]
Chang, Yang Sil [1 ]
Nakamura, Takafumi [1 ]
Nakai, Yuichiro [1 ]
Shimoya, Koichiro [1 ]
机构
[1] Kawasaki Med Sch, Dept Obstet & Gynecol, Kurashiki, Okayama 7010192, Japan
[2] Osaka Univ, Grad Sch Med, Dept Obstet & Gynecol, Osaka, Japan
关键词
Prostaglandin; Preterm labor; Oxidative stress; Myometrium; N-Acetylcysteine; 4-Hydroxy-2-nonenal; Cyclooxygenase-2; OXIDATIVE STRESS; PRETERM LABOR; HUMAN PLACENTA; CELLS SHOW; LIPOPOLYSACCHARIDE; INTERLEUKIN-8; INFLAMMATION; ATTENUATION; METABOLISM; PREGNANCY;
D O I
10.1159/000322393
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Background: Chorioamnionitis is one of the important causes of preterm labor. Preterm labor with chorioamnionitis is associated with oxidative stress. We reported that 4-hydroxy-2-nonenal (4-HNE), a major end product of oxidative fatty acid metabolism, is accumulated in the placenta with chorioamnionitis. The aim of this study was to confirm the effect of 4-HNE on cyclooxygenase-2 (COX-2) and prostaglandin (PG) induction in the uterine myometrial tissues. We also examined the effect of N-acetylcysteine (NAC) on 4-HNE-induced COX-2 expression. Methods: Uterine myometrial tissues were obtained from 5 patients. One of them underwent elective cesarean section without labor, and 4 of them underwent hysterectomy because of placental previa or atonic bleeding. We stimulated the uterine myometrial tissues with 4-HNE. In addition, the tissues were pretreated with NAC before 4-HNE treatment. The expression of COX-2 mRNA was observed by real-time PCR. PGE2 and prostacyclin release into the supernatants of the tissue cultures was measured by ELISA. Results: 4-HNE induced the COX-2 mRNA expression and PGE2 production in the uterine myometrial tissue culture in a dose-dependent and time-dependent manner. NAC inhibited 4-HNE-induced COX-2 expression. Conclusion: 4-HNE may play an important role in preterm labor. NAC might be protective against preterm labor under oxidative stress. Copyright (C) 2011 S. Karger AG, Basel
引用
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页码:37 / 42
页数:6
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