Crucial role for the LSP1-myosin1e bimolecular complex in the regulation of Fcγ receptor-driven phagocytosis

被引：26

作者：

Maxeiner, Sebastian ^{[1
]}

Shi, Nian ^{[1
]}

Schalla, Carmen ^{[1
]}

Aydin, Guelcan ^{[1
]}

Hoss, Mareike ^{[2
]}

Vogel, Simon ^{[3
]}

Zenke, Martin ^{[1
]}

Sechi, Antonio S. ^{[1
]}

机构：

[1] Uniklin RWTH Aachen, Dept Cell Biol, Inst Biomed Engn, D-52074 Aachen, Germany

[2] Uniklin RWTH Aachen, Electron Microscopy Facil, D-52074 Aachen, Germany

[3] Fraunhofer Inst Mol Biol & Appl Ecol, D-52074 Aachen, Germany

来源：

MOLECULAR BIOLOGY OF THE CELL | 2015年 / 26卷 / 09期

关键词：

LYMPHOCYTE-SPECIFIC PROTEIN-1; NEUTROPHIL ACTIN DYSFUNCTION; LEUKOCYTE-SPECIFIC PROTEIN-1; R-MEDIATED PHAGOCYTOSIS; MYOSIN; 1E; CA-2+-BINDING PROTEIN; NAD; 47/89; P50; LSP1; T-CELLS; CYTOSKELETON;

D O I：

10.1091/mbc.E14-05-1005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Actin cytoskeleton remodeling is fundamental for Fc gamma receptor-driven phagocytosis. In this study, we find that the leukocyte-specific protein 1 (LSP1) localizes to nascent phagocytic cups during Fc gamma receptor-mediated phagocytosis, where it displays the same spatial and temporal distribution as the actin cytoskeleton. Down-regulation of LSP1 severely reduces the phagocytic activity of macrophages, clearly demonstrating a crucial role for this protein in Fc gamma receptor-mediated phagocytosis. We also find that LSP1 binds to the class I molecular motor myosin1e. LSP1 interacts with the SH3 domain of myosin1e, and the localization and dynamics of both proteins in nascent phagocytic cups mirror those of actin. Furthermore, inhibition of LSP1-myosin1e and LSP1-actin interactions profoundly impairs pseudopodial formation around opsonized targets and their subsequent internalization. Thus the LSP1-myosin1e bimolecular complex plays a pivotal role in the regulation of actin cytoskeleton remodeling during Fc gamma receptor-driven phagocytosis.

引用

页码：1652 / 1664

页数：13

共 50 条

[1] Phosphoinositide-3-kinase-independent contractile activities associated with Fcγ-receptor-mediated phagocytosis and macropinocytosis in macrophages [J].

Araki, N ;

Hatae, T ;

Furukawa, A ;

Swanson, JA .

JOURNAL OF CELL SCIENCE, 2003, 116 (02) :247-257

[2]

Carballo E, 1996, J IMMUNOL, V156, P1709

[3] Myosin 1E coordinates actin assembly and cargo trafficking during clathrin-mediated endocytosis [J].

Cheng, Jackie ;

Grassart, Alexandre ;

Drubin, David G. .

MOLECULAR BIOLOGY OF THE CELL, 2012, 23 (15) :2891-2904

[4]

COATES TD, 1991, BLOOD, V78, P1338

[5]

Coppolino MG, 2001, J CELL SCI, V114, P4307

[6] Myosin X is a downstream effector of PI(3)K during phagocytosis [J].

Cox, D ;

Berg, JS ;

Cammer, M ;

Chinegwundoh, JO ;

Dale, BM ;

Cheney, RE ;

Greenberg, S .

NATURE CELL BIOLOGY, 2002, 4 (07) :469-477

[7] The motor protein myosin 1G functions in FcγR-mediated phagocytosis [J].

Dart, Anna E. ;

Tollis, Sylvain ;

Bright, Michael D. ;

Frankel, Gad ;

Endres, Robert G. .

JOURNAL OF CELL SCIENCE, 2012, 125 (24) :6020-6029

[8] Myole Binds Anionic Phospholipids with High Affinity [J].

Feeser, Elizabeth A. ;

Ignacio, Cherry Mae G. ;

Krendel, Mira ;

Ostap, E. Michael .

BIOCHEMISTRY, 2010, 49 (43) :9353-9360

[9] Contribution of Ena/VASP proteins to intracellular motility of Listeria requires phosphorylation and proline-rich core but not F-actin binding or multimerization [J].

Geese, M ;

Loureiro, JJ ;

Bear, JE ;

Wehland, J ;

Gertler, FB ;

Sechi, AS .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (07) :2383-2396

[10] Myosin 1E localizes to actin polymerization sites in lamellipodia, affecting actin dynamics and adhesion formation [J].

Gupta, Prabuddha ;

Gauthier, Nils C. ;

Yu Cheng-Han ;

Yuan Zuanning ;

Pontes, Bruno ;

Ohmstede, Malte ;

Martin, Rene ;

Knoelker, Hans-Joachim ;

Doebereiner, Hans-Guenther ;

Krendel, Mira ;

Sheetz, Michael .

BIOLOGY OPEN, 2013, 2 (12) :1288-1299

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