LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model

被引：16

作者：

Abdel-Salam, Mostafa A. L. ^{[1
]}

Pinto, Barbara ^{[1
]}

Cassali, Geovanni ^{[2
]}

Bueno, Lilian ^{[3
]}

Pegas, Gabriela ^{[2
]}

Oliveira, Fabricio ^{[2
]}

Silva, Irismara ^{[4
]}

Klein, Andre ^{[4
]}

de Souza-Fagundes, Elaine Maria ^{[1
]}

de Lima, Maria Elena ^{[5
]}

Carvalho-Tavares, Juliana ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Fisiol & Biofis, Programa Posgrad Ciencias Biol Fisiol & Farmacol, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Patol Geral, BR-31270901 Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil

[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Farmacol, Programa Posgrad Ciencias Biol Fisiol & Farmacol, BR-31270901 Belo Horizonte, MG, Brazil

[5] Fac Santa Casa Belo Horizonte, Programa Posgrad Med Biomed, BR-30110005 Belo Horizonte, MG, Brazil

来源：

ANTIBIOTICS-BASEL | 2021年 / 10卷 / 09期

关键词：

breast cancer; LyeTx I-b peptide; immunomodulation; leukocyte recruitment; cytokines; anticancer activity of LyeTx I-b; Lycosa erythrognatha; ANTIMICROBIAL PEPTIDES; CHEMOTHERAPY; APOPTOSIS; INFLAMMATION; CARCINOMA; MIGRATION; EFFICACY; IMMUNITY; THERAPY; CELLS;

D O I：

10.3390/antibiotics10091136

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Cationic anticancer peptides have exhibited potent anti-proliferative and anti-inflammatory effects in neoplastic illness conditions. LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on the effects of LyeTxI-b on a 4T1 mouse mammary carcinoma model. Mice with a palpable tumor in the left flank were subcutaneously or intratumorally injected with LyeTx I-b (5 mg/kg), which significantly decreased the tumor volume and metastatic nodules. Histological analyses showed a large necrotic area in treated primary tumors compared to the control. LyeTxI-b reduced tumor growth and lung metastasis in the 4T1 mouse mammary carcinoma model with no signs of toxicity in healthy or cancerous mice. The mechanism of action of LyeTx I-b on the 4T1 mouse mammary carcinoma model was evaluated in vitro and is associated with induction of apoptosis and cell proliferation inhibition. Furthermore, LyeTx I-b seems to be an efficient regulator of the 4T1 tumor microenvironment by modulating several cytokines, such as TGF-beta, TNF-alpha, IL-1 beta, IL-6, and IL-10, in primary tumor and lung, spleen, and brain. LyeTx I-b also plays a role in leukocytes rolling and adhesion into spinal cord microcirculation and in the number of circulating leukocytes. These data suggest a potent antineoplastic efficacy ofLyeTx I-b.

引用

页数：28