Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp.

被引：15

作者：

Jawad, A ^{[1
]}

Snelling, AM ^{[1
]}

Heritage, J ^{[1
]}

Hawkey, PM ^{[1
]}

机构：

[1] Univ Leeds, Dept Microbiol, Leeds LS2 9JT, W Yorkshire, England

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 165卷 / 02期

关键词：

Acinetobacter; recA; restriction fragment length polymorphism; amplified ribosomal DNA restriction analysis; genospecies;

D O I：

10.1016/S0378-1097(98)00302-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well-characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of recA PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the recA gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of recA RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp, to be determined. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：357 / 362

页数：6

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