Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

被引：20

作者：

Chae, Soomin ^{[1
]}

Lee, Hyun-Kyung ^{[1
]}

Kim, Yoo-Kyung ^{[1
]}

Sim, Hyo Jung ^{[2
]}

Ji, Yoorim ^{[1
]}

Kim, Chowon ^{[1
]}

Ismail, Tayaba ^{[1
]}

Park, Jeen-Woo ^{[1
]}

Kwon, Oh-Shin ^{[1
]}

Kang, Beom-Sik ^{[1
]}

Lee, Dong-Seok ^{[1
]}

Bae, Jong-Sup ^{[3
]}

Kim, Sang-Hyun ^{[4
]}

Min, Kyoung-Jin ^{[5
]}

Kwon, Taeg Kyu ^{[5
]}

Park, Mae-Ja ^{[6
]}

Han, Jin-Kwan ^{[7
]}

Kwon, Taejoon ^{[2
]}

Park, Tae-Joo ^{[2
]}

Lee, Hyun-Shik ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Sch Life Sci, Plus KNU Creat BioRes Grp BK21, KNU Ctr Nonlinear Dynam,Coll Nat Sci, Daegu 41566, South Korea

[2] Ulsan Natl Inst Sci & Technol, Sch Life Sci, Ulsan 44919, South Korea

[3] Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu 41566, South Korea

[4] Kyungpook Natl Univ, Dept Pharmacol, Coll Med, Daegu 41944, South Korea

[5] Keimyung Univ, Sch Med, Dept Immunol, Daegu 42601, South Korea

[6] Kyungpook Natl Univ, Dept Anat, Coll Med, Daegu 41944, South Korea

[7] Pohang Univ Sci & Technol, Dept Life Sci, Pohang 37673, South Korea

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

新加坡国家研究基金会;

关键词：

PLANAR CELL POLARITY; XENOPUS PRONEPHROS; SUBCELLULAR-LOCALIZATION; KIDNEY DEVELOPMENT; EXPRESSION; GENE; TUBULOGENESIS; SPECIFICATION; INDUCTION; SYSTEMS;

D O I：

10.1038/s41598-017-09262-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS ( H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.

引用

页数：10

共 46 条

[1] Inflammatory Cytokines and Reactive Oxygen Species as Mediators of Chronic Kidney Disease-Related Vascular Calcification [J].