Impacts of nitrogen addition on the carbon balance in a temperate semiarid grassland ecosystem

Understanding carbon (C) cycling and sequestration in vegetation and soils, and their responses to nitrogen (N) deposition, is important for quantifying ecosystem responses to global climate change. Here, we describe a 2-year study of the C balance in a temperate grassland in northern China. We measured net ecosystem CO2 exchange (NEE), net ecosystem production (NEP), and C sequestration rates in treatments with N addition ranging from 0 to 25 g N m(-2) year(-1). High N addition significantly increased ecosystem C sequestration, whose rates ranged from 122.06 g C m(-2) year(-1) (control) to 259.67 g C m(-2) year(-1) (25 g N). Cumulative NEE during the growing season decreased significantly at high and medium N addition, with values ranging from -95.86 g C m(-2) (25 g N) to 0.15 g C m(-2) (5 g N). Only the highest N rate increased significantly cumulative soil microbial respiration compared with the control in the dry 2014 growing season. High N addition significantly increased net primary production (NPP) and NEP in both years, and NEP ranged from -5.83 to 128.32 g C m(-2). The C input from litter decomposition was significant and must be quantified to accurately estimate NPP. Measuring C sequestration and NEP together may allow tracking of the effects of N addition on grassland C budgets. Overall, adding 25 or 10 g N m(-2) year(-1) improved the CO2 sink of the grassland ecosystem, and increased grassland C sequestration.