Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings

被引:8
作者
He, Jiahui [1 ,2 ,3 ]
Zhang, Yukun [1 ,2 ,3 ]
Hu, Zezhong [1 ,2 ,3 ]
Zhang, Luxuan [4 ]
Shao, Guanming [1 ,2 ,3 ]
Xie, Zi [1 ,2 ,3 ]
Nie, Yu [1 ,2 ,3 ]
Li, Wenxue [1 ,2 ,3 ]
Li, Yajuan [1 ,2 ,3 ]
Chen, Liyi [1 ,2 ,3 ]
Huang, Benli [1 ,2 ,3 ]
Chu, Fengsheng [1 ,2 ,3 ]
Feng, Keyu [1 ,2 ,3 ,5 ]
Lin, Wencheng [1 ,2 ,3 ,5 ]
Li, Hongxin [1 ,2 ,3 ,5 ]
Chen, Weiguo [1 ,2 ,3 ,5 ]
Zhang, Xinheng [1 ,2 ,3 ,5 ]
Xie, Qingmei [1 ,2 ,3 ,5 ]
机构
[1] South China Agr Univ, Coll Anim Sci, Heyuan Branch, Guangdong Prov Lab Lingnan Modern Agr Sci & Techn, Guangzhou 510642, Peoples R China
[2] Guangdong Engn Res Ctr Vector Vaccine Anim Virus, Guangzhou 510642, Peoples R China
[3] South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Peoples R China
[4] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
[5] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Peoples R China
来源
VIRUSES-BASEL | 2022年 / 14卷 / 07期
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
waterfowl parvovirus; inflammation; ileac microflora; oxidative stress; molecular characterization; ORIGIN GOOSE PARVOVIRUS; OXIDATIVE STRESS; GENE-EXPRESSION; GENOMIC CHARACTERIZATION; INFLAMMATORY RESPONSES; INTESTINAL-MUCOSA; VIRUS-INFECTION; ENTEROCOCCUS; MICROBIOTA; IDENTIFICATION;
D O I
10.3390/v14071471
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Waterfowl parvovirus (WPFs) has multiple effects on the intestinal tract, but the effects of recombinant Muscovy duck parvovirus (rMDPV) have not been elucidated. In this study, 48 one-day-old Muscovy ducklings were divided into an infected group and a control group. Plasma and ileal samples were collected from both groups at 2, 4, 6, and 8 days post-infection (dpi), both six ducklings at a time. Next, we analyzed the genomic sequence of the rMDPV strain. Results showed that the ileal villus structure was destroyed seriously at 4, 6, 8 dpi, and the expression of ZO-1, Occludin, and Claudin-1 decreased at 4, 6 dpi; 4, 6, 8 dpi; and 2, 6 dpi, respectively. Intestinal cytokines IFN-alpha, IL-1 beta and IL-6 increased at 6 dpi; 8 dpi; and 6, 8 dpi, respectively, whereas IL-2 decreased at 6, 8 dpi. The diversity of ileal flora increased significantly at 4 dpi and decreased at 8 dpi. The bacteria Ochrobactrum and Enterococcus increased and decreased at 4, 8 dpi; 2, 4 dpi, respectively. Plasma MDA increased at 2 dpi, SOD, CAT, and T-AOC decreased at 2, 4, 8 dpi; 4, 8 dpi; and 4, 6, 8 dpi, respectively. These results suggest that rMDPV infection led to early intestinal barrier dysfunction, inflammation, ileac microbiota disruption, and oxidative stress.
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页数:19
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