Characterization of monoclonal antibodies produced by hybridoma cell lines prepared against hepatitis B virus X protein

被引：0
|
作者：
Chung, MK [1 ]
Lee, HG [1 ]
Oh, ES [1 ]
Min, SS [1 ]
Lee, HK [1 ]
Lee, TG [1 ]
Lim, JS [1 ]
Kim, CM [1 ]
Park, SN [1 ]
机构：
[1] Korea Res Inst Biosci & Biotechnol, Virus Oncol Res Unit, Mol & Cell Biol Res Div, Taejon 305600, South Korea
来源：
ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 9 | 1998年
关键词：
hepatitis B virus; HBx protein; monoclonal antibody; serological response;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The X gene of HBV encodes HBx protein which is required for virus replication and has been implicated in virus mediated liver oncogenesis, However, the molecular mechanism of HBx activity and its possible influence on cell proliferation have to be solved further in detail. To understand more about the immune responses against HBV and to make tools useful for preparing diagnostic and therapeutic agents, we have produced monoclonal antibodies against HBx protein. Eight independent hybridoma cell lines including X-01A were isolated for this purified HBx protein. The isotype of the antibody produced from X-01A hybridoma cell line was IgG1. Some HBs-positive sera tested contained antibodies that specifically recognized the HBx protein. We conclude that the HBx protein is antigenic in some patients.
引用
收藏
页码：379 / 384
页数：6
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