Investigation of Multiple Pathogens in Black-Spotted Dendrobium officinale Based on Culture-Independent and Dependent Methods

被引：0

作者：

Han, Xiaoying ^{[1
]}

Dai, Yihong ^{[1
]}

Hu, Miao ^{[1
]}

Xiao, Rong ^{[1
]}

Zhang, Shuaishuai ^{[1
]}

Li, Jin ^{[1
]}

Wang, Zhanqi ^{[2
]}

Hu, Xiufang ^{[1
]}

Gu, Zhouhang ^{[1
]}

机构：

[1] Zhejiang Sci Tech Univ, Coll Life Sci & Med, Key Lab Plant Secondary Metab & Regulat Zhejiang, Hangzhou 310018, Zhejiang, Peoples R China

[2] Huzhou Univ, Coll Life Sci, Key Lab Vector Biol & Pathogen Control Zhejiang P, Huzhou 313000, Zhejiang, Peoples R China

来源：

JOURNAL OF BIOBASED MATERIALS AND BIOENERGY | 2020年 / 14卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Dendrobium officinale; Black Spot Disease; Cladosporium; Alternaria; Amplicon Sequencing; 1ST REPORT; LEAF-SPOT; DISEASE; ALTERNATA; BACTERIAL; L;

D O I：

10.1166/jbmb.2020.1959

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Black spot disease which significantly reduces the quality of medicine plants is quite common in Dendrobium officinale fields. In this study, GXDF33, a strain of Alternaria, together with the reported pathogen Cladosporium, was isolated from symptomatic leaves. The in vitro pathogenicity tests on seedlings showed GXDF33 can cause black spot disease. Based on quantitative real-time polymerase chain reaction (qPCR) result, it was found that the biomass of both Cladosporium and Alternaria increased as the symptom went severer. According to amplicon sequencing data for the symptomatic samples from Guangxi and Zhejiang Provinces in China, Cladosporium and Alternaria were both common genera in symptomatic plants. To our knowledge, this is the first report unveiling the relationship between black spot disease of a officirrale and Ciadosportum as well as Alternaria.

引用

页码：249 / 257

页数：9

共 31 条

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