Unhairing wastewater treatment by Bacillus pumilus and Bacillus cereus

被引:4
|
作者
Mlaik, Najoua [1 ]
Bouzid, Jalel [1 ]
Ben Hassan, Ines [2 ]
Woodward, Steve [3 ]
Belbahri, Lassaad [4 ]
Mechichi, Tahar [5 ]
机构
[1] ENIS, Lab Eau Energie & Environm, Sfax, Tunisia
[2] ENIS, LRAE, Sfax, Tunisia
[3] Univ Aberdeen, Dept Plant & Soil Sci, Inst Biol & Environm Sci, Aberdeen AB24 3UU, Scotland
[4] Univ Neuchatel, Lab Soil Biol, CH-2009 Neuchatel, Switzerland
[5] ENIS, Lab Genie Enzymat & Microbiol, Sfax, Tunisia
关键词
Keratinase; Bacillus cereus; Tannery wastewater; Protease; Bacillus pumilus; Biological treatment; KERATINOLYTIC ACTIVITY; KERATINASE; PROTEASE; FEATHER; PROTEINASE; EFFLUENTS; DESIGN;
D O I
10.1080/19443994.2014.883330
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
Two proteolytic bacteria Bacillus pumilus and Bacillus cereus were isolated from an aerobic tannery effluent treatment tank. Both strains were characterized and used for the treatment of unhairing wastewater during batch experiments. Chemical oxygen demand (COD), biomass production and protease activity were determined during the incubation period. The maximum COD removals were of 41 and 44% after 10 days incubation in B. cereus and B. pumilus, respectively. Optimum pH and temperature for protease and keratinase production by both strains were also investigated. Results showed that the optimum temperature and pH of protease production in the cultures of B. pumilus and B. cereus were of 30 degrees C and 9.0, respectively. Under these optimum conditions, proteolytic activities were 283.8 and 515.8 U/mL in the cultures of B. cereus and B. pumilus, respectively. Optimum keratinase production by cultures of both bacteria occurred at the same temperature and pH of the protease production.
引用
收藏
页码:683 / 689
页数:7
相关论文
共 50 条
  • [41] ANALYTICAL METHODS FOR BACILLUS-CEREUS AND OTHER BACILLUS SPECIES
    SHINAGAWA, K
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1990, 10 (02) : 125 - 142
  • [42] Expression of Bacillus pumilus keratinase rK27 in Bacillus subtilis: enzyme application for developing renewable flocculants from bone meal
    Rajput, Rinky
    Gupta, Rani
    ANNALS OF MICROBIOLOGY, 2014, 64 (03) : 1257 - 1266
  • [43] Treatment of Bacillus cereus endophthalmitis with endoscopy-assisted vitrectomy
    Pan, Qintuo
    Liu, Yanhua
    Wang, Ruixi
    Chen, Tianyu
    Yang, Zhengwei
    Deng, Yuxuan
    Zhao, Zhenquan
    Hu, Xuting
    Chen, Xiaomeng
    Wei, Wenlong
    Zhang, Zongduan
    Wang, Yuqin
    Zheng, Jingwei
    Ke, Zhisheng
    MEDICINE, 2017, 96 (50)
  • [44] Salt stress represses production of extracellular proteases in Bacillus pumilus
    Liu, R. F.
    Huang, C. L.
    Feng, H.
    GENETICS AND MOLECULAR RESEARCH, 2015, 14 (02) : 4939 - 4948
  • [45] Stability studies of protease from Bacillus cereus BG1
    Ghorbel, B
    Sellami-Kamoun, A
    Nasri, M
    ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (05) : 513 - 518
  • [46] Conversion of squid pens to chitosanases and dye adsorbents via Bacillus cereus
    Wang, San-Lang
    Yu, Hao-Ting
    Tsai, Min-Hsiung
    Doan, Chien Thang
    Van Bon Nguyen
    Van Chung Do
    Anh Dzung Nguyen
    RESEARCH ON CHEMICAL INTERMEDIATES, 2018, 44 (08) : 4903 - 4911
  • [47] Cloning and characterization of a thermostable detergent-compatible recombinant keratinase from Bacillus pumilus KS12
    Rajput, Rinky
    Sharma, Richa
    Gupta, Rani
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2011, 58 (02) : 109 - 118
  • [48] Regulatory Characteristics of Bacillus pumilus Protease Promoters
    Anna A. Toymentseva
    Thorsten Mascher
    Margarita R. Sharipova
    Current Microbiology, 2017, 74 : 550 - 559
  • [49] Novel Feather Degrading Keratinases from Bacillus cereus Group: Biochemical, Genetic and Bioinformatics Analysis
    Almahasheer, Arwa Ali
    Mahmoud, Amal
    El-Komy, Hesham
    Alqosaibi, Amany I.
    Aktar, Sultan
    AbdulAzeez, Sayed
    Borgio, J. Francis
    MICROORGANISMS, 2022, 10 (01)
  • [50] Features of gene expression of Bacillus pumilus metalloendopeptidase
    Rudakova, N. L.
    Sabirova, A. R.
    Balaban, N. P.
    Tikhonova, A. O.
    Sharipova, M. R.
    BIOCHEMISTRY-MOSCOW, 2016, 81 (08) : 884 - 891