Localization of the pituitary adenylate cyclase-activating polypeptide receptor and its mRNA in the rat adrenal medulla

被引：11

作者：

Shioda, S

Shimoda, Y

Hori, T

Mizushima, H

Ajiri, T

Funahashi, H

Ohtaki, K

Ryushi, T

机构：

[1] Showa Univ, Sch Med, Dept Anat, Shinagawa Ku, Tokyo 1428555, Japan

[2] Tokyo Metropolitan Univ, Grad Sch Sci, Dept Kinesiol, Tokyo 1920364, Japan

来源：

NEUROSCIENCE LETTERS | 2000年 / 295卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

adrenal medulla; rat; pituitary adenylate cyclase-activating peptide receptor; immunohistochemistry; in situ hybridization;

D O I：

10.1016/S0304-3940(00)01595-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We examined the localization of the pituitary adenylate cyclase-activating peptide (PACAP) receptor (PAC1-R) and its mRNA with immunocytochemistry and in situ hybridization, respectively. PAC1-R immunoreactivity and its transcript were detected in both chromaffin cells and ganglion cells but not detected in the adrenal cortex. In addition, strong PAC1-R immunoreactivity was found beneath the plasma membrane of the immunoreactive medullary cells. Electron microscopic immunocytochemistry revealed that PAC1-R was predominantly expressed in adrenaline-containing cells. This report supports the notion that PACAP is an activator and modulator of catecholamine secretion as well as synthesis in the adrenal medulla. (C) 2000 Published by Elsevier Science Ireland Ltd.

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页码：81 / 84

页数：4

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