Rapid Ultrathin Slice Plastination of Embalmed Specimens With Minimal Tissue Loss

被引：13

作者：

Soal, S. ^{[1
]}

Pollard, M. ^{[1
]}

Burland, G. ^{[2
]}

Lissaman, R. ^{[1
]}

Wafer, M. ^{[1
]}

Stringer, M. D. ^{[1
]}

机构：

[1] Univ Otago, Otago Sch Med Sci, Dept Anat & Struct Biol, Dunedin 9054, New Zealand

[2] Univ Sydney, Fac Vet Sci, Sydney, NSW 2006, Australia

来源：

CLINICAL ANATOMY | 2010年 / 23卷 / 05期

关键词：

submacroscopic anatomy; 3-D reconstruction; decalcification; RECONSTRUCTION; ANKLE; BONE;

D O I：

10.1002/ca.20972

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

A modified technique of producing 1 mm (ultrathin) E12 plastinated slices of tissue specimens from embalmed cadavers for anatomical teaching and research is described. Specimens up to 150 mm in length and width were embedded in polyurethane foam and serially sectioned using an OMAS C300 food slicer. Individual slices were then processed by cold dehydration, degreasing, resin impregnation, mounting and curing. Bone-containing specimens were first decalcified. Tissue slices could be cut with remarkable accuracy (0.98 +/- 0.01 mm per slice) and minimal tissue loss (less than 2% per slice). The entire production process could be achieved in 25 days and was associated with tissue volume shrinkage of no more than 6%. These results demonstrate a relatively rapid method of producing ultrathin E12 slices with minimal tissue loss. The technique may be particularly applicable to submacroscopic morphologic studies and three-dimensional reconstruction in clinical anatomy. Clin. Anat. 23:539-544, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：539 / 544

页数：6

共 19 条

[1] Sectional depiction of the pelvic floor by CT, MR imaging and sheet plastination: computer-aided correlation and 3D model [J].

Beyersdorff, D ;

Schiemann, T ;

Taupitz, M ;

Kooijman, H ;

Hamm, B ;

Nicolas, V .

EUROPEAN RADIOLOGY, 2001, 11 (04) :659-664

[2]

COOK P, 1997, INT SOC PLASTINATION, V12, P17

[3]

Fasel J., 1988, J INT SOC PLAST, V2, P4

[4]

FRITSCH H, 1991, ANAT ANZEIGER, V173, P161

[5] The different growth zones of the fetal foot [J].

Gruber, H ;

Brenner, E ;

Schmitt, O ;

Fritsch, H .

ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 2001, 183 (03) :267-274

[6]

Johnson G., 2000, Journal of the International Society for Plastination, V15, P10

[7] Plastination and computerized 3D reconstruction of the temporal bone [J].

Qiu, MG ;

Zhang, SX ;

Liu, ZJ ;

Tan, LW ;

Wang, YS ;

Deng, JH ;

Tang, ZS .

CLINICAL ANATOMY, 2003, 16 (04) :300-303

[8] Computerized 3D-reconstructions of the ligaments of the lateral aspect of ankle and subtalar joints [J].

Sha, Y ;

Zhang, SX ;

Liu, ZJ ;

Tan, LW ;

Wu, XY ;

Wan, YS ;

Deng, JH ;

Tang, ZS .

SURGICAL AND RADIOLOGIC ANATOMY, 2001, 23 (02) :111-114

[9]

Sora M. C., 2004, J INT SOC PLASTINATI, V19, P22

[10]

SORA MC, 1997, J INT SOC PLASTINATI, V22, P31

← 1 2 →