Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2

被引：22

作者：

Ferreira, Graziela Batista

Evangelista, Alex Ferreira

Severo Junio, Joao Baptista

de Souza, Roberto Rodrigues

Curvelo Santana, Jose Carlos

Tambourgi, Elias Basile

Jordao, Elizabete

机构：

[1] Univ Fed Sergipe, Dept Engn Quim, BR-49100000 Sao Cristovao, SE, Brazil

[2] Univ Estadual Campinas, Fac Engn Quim, BR-13083970 Campinas, SP, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2007年 / 50卷 / 03期

关键词：

partitioning; optimization; aqueous two-phase systems; maize malt; PEG; 6000; CaCl2;

D O I：

10.1590/S1516-89132007000300020

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable for continuous aqueous two phase purification due to the stability of proteins (e.g. alpha and beta-amylases) and could increase their content into middle.

引用

页码：557 / 563

页数：7

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