Mesoporous Silica Promotes Osteogenesis of Human Adipose-Derived Stem Cells Identified by a High-Throughput Microfluidic Chip Assay

被引：5

作者：

Chen, Xin ^{[1
]}

Wang, Chao ^{[2
]}

Hao, Min ^{[1
]}

Zhao, Hang ^{[1
]}

Xia, He ^{[1
]}

Yu, Liyang ^{[1
]}

Li, Dong ^{[3
]}

Qiu, Jichuan ^{[1
]}

Li, Haijun ^{[4
]}

Han, Lin ^{[2
]}

Sang, Yuanhua ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China

[2] Shandong Univ, Inst Marine Sci & Technol, Tsingdao 266237, Peoples R China

[3] Shandong Univ, Qilu Hosp, Cryomed Lab, Jinan 250012, Peoples R China

[4] Shandong Univ, Qilu Hosp, Dept Geriatr Med, Key Lab Cardiovasc Prote Shandong Prov, Jinan 250012, Peoples R China

来源：

PHARMACEUTICS | 2022年 / 14卷 / 12期

关键词：

osteogenic differentiation; human adipose-derived stem cells; mesoporous silica; microfluidic detection; BONE-MARROW; IN-VITRO; DIFFERENTIATION; HYDROXYAPATITE;

D O I：

10.3390/pharmaceutics14122730

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Silicon-derived biomaterials are conducive to regulating the fate of osteo-related stem cells, while their effects on the osteogenic differentiation of human adipose-derived stem cells (hADSCs) remain inconclusive. Mesoporous silica (mSiO(2)) is synthesized in a facile route that exhibited the capability of promoting osteogenic differentiation of hADSCs. The metabolism of SiO2 in cells is proposed according to the colocalization fluorescence analysis between lysosomes and nanoparticles. The released silicon elements promote osteogenic differentiation. The detection of secretory proteins through numerous parallel experiments performed via a microfluidic chip confirms the positive effect of SiO2 on the osteogenic differentiation of hADSCs. Moreover, constructed with superparamagnetic iron oxide (Fe3O4), the magnetic nanoparticles (MNPs) of Fe3O4@mSiO(2) endow the cells with magnetic resonance imaging (MRI) properties. The MNP-regulated osteogenic differentiation of autologous adipose-derived stem cells provides considerable clinical application prospects for stem cell therapy of bone tissue repair with an effective reduction in immune rejection.

引用

页数：19

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