Antibiotic Resistance and Extended-Spectrum β-Lactamases in Isolated Bacteria from Seawater of Algiers Beaches (Algeria)

被引:28
作者
Alouache, Souhila [1 ,2 ]
Kada, Mohamed [2 ]
Messai, Yamina [1 ]
Estepa, Vanesa [3 ]
Torres, Carmen [3 ]
Bakour, Rabah [1 ]
机构
[1] Univ Sci & Technol Houari Boumediene, Fac Biol Sci, Biol Cellulaire & Mol Lab, Bab Ezzouar 16111, Alger, Algeria
[2] Ecole Natl Super Sci Mer & Amenagement Littoral, Algiers, Algeria
[3] Univ La Rioja, Area Bioquim & Biol Mol, Logrono 26006, Spain
关键词
antibiotic resistance; bacteria; seawater; beach; GRAM-NEGATIVE BACTERIA; WASTE-WATER TREATMENT; CTX-M; PSEUDOMONAS-AERUGINOSA; GENES; PLASMID; PREVALENCE; SEDIMENT; ENTEROBACTERIACEAE; SUSCEPTIBILITY;
D O I
10.1264/jsme2.ME11266
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in Algiers. The most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. According to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved from anthropogenic influence, resistance rates were lowest (amoxicillin, 1.5% and 16%; ticarcillin, 0.8% and 2.6%). Thirty-four bacteria resistant to imipenem (n=14) or cefotaxime (n=20) were identified as Pseudomonas aeruginosa (n=15), Pseudomonas fluorescens (7), Stenotrophomonas maltophilia (4), Burkholderia cepacia (2), Bordetella sp. (1), Pantoea sp. (1), Acinetobacter baumannii (1), Chryseomonas luteola (1), Ochrobactrum anthropi (1) and Escherichia coli (1). Screening for extended spectrum beta-lactamase showed the presence of CTX-M-15 beta-lactamase in the E. coli isolate, and the encoding gene was transferable in association with the IncI1 plasmid of about 50 kbp. Insertion sequence ISEcp1B was located upstream of the CTX-M-15 gene. This work showed a significant level of resistance to antibiotics, mainly among environmental saprophytic bacteria. Transmissible CTX-M-15 was detected in E. coli; this may mean that contamination of the environment by resistant bacteria may cause the spread of resistance genes.
引用
收藏
页码:80 / 86
页数:7
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