The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

被引:14
作者
Jirakova, Klara [1 ]
Seneklova, Monika [1 ,2 ]
Jirak, Daniel [3 ,4 ]
Turnovcova, Karolina [1 ]
Vosmanska, Magda [5 ]
Babic, Michal [6 ]
Horak, Daniel [6 ]
Veverka, Pavel [7 ]
Jendelova, Pavla [1 ,2 ]
机构
[1] Acad Sci Czech Republ, Inst Expt Med, Dept Neurosci, Videnska 1083, Prague 14220 4, Czech Republic
[2] Charles Univ Prague, Fac Med 2, Dept Neurosci, Prague, Czech Republic
[3] Inst Clin & Expt Med, Radiodiagnost & Intervent Radiol Dept, MR Unit, Prague, Czech Republic
[4] Charles Univ Prague, Fac Med 1, Inst Biophys & Informat, Dept Biophys, Prague, Czech Republic
[5] Univ Chem & Technol, Dept Analyt Chem, Prague, Czech Republic
[6] Acad Sci Czech Republ, Inst Macromol Chem, Dept Polymer Particles, Prague, Czech Republic
[7] ASCR, Inst Phys, Dept Magnet & Superconductors, Prague, Czech Republic
来源
INTERNATIONAL JOURNAL OF NANOMEDICINE | 2016年 / 11卷
关键词
neural precursors; magnetic resonance imaging; cell differentiation; superparamagnetic iron oxide nanoparticles; ferrites; FUNCTIONAL DOPAMINERGIC-NEURONS; RESONANCE-IMAGING TRACKING; ZINC FERRITE NANOPARTICLES; MRI VISUALIZATION; HIGHLY EFFICIENT; GENERATION; DISEASE; BRAIN; MODEL;
D O I
10.2147/IJN.S116171
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated gamma-Fe2O3) and studied their effect on proliferation and neuronal differentiation. Materials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. Results: Cell proliferation was not affected by PLL-coated gamma-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated gamma-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. Conclusion: Our results show that cells labeled with PLL-coated gamma-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.
引用
收藏
页码:6267 / 6281
页数:15
相关论文
共 50 条
  • [1] Midbrain dopamine neuron differentiation: Factors and fates
    Abeliovich, Asa
    Hammond, Rachel
    [J]. DEVELOPMENTAL BIOLOGY, 2007, 304 (02) : 447 - 454
  • [2] Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labeling
    Babic, Michal
    Horak, Daniel
    Trchova, Miroslava
    Jendelova, Pavla
    Glogarova, Katerina
    Lesny, Petr
    Herynek, Vit
    Hajek, Milan
    Sykova, Eva
    [J]. BIOCONJUGATE CHEMISTRY, 2008, 19 (03) : 740 - 750
  • [3] Tracking stem cells using magnetic nanoparticles
    Berman, Stacey M. Cromer
    Walczak, Piotr
    Bulte, Jeff W. M.
    [J]. WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY, 2011, 3 (04) : 343 - 355
  • [4] Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    Chambers, Stuart M.
    Fasano, Christopher A.
    Papapetrou, Eirini P.
    Tomishima, Mark
    Sadelain, Michel
    Studer, Lorenz
    [J]. NATURE BIOTECHNOLOGY, 2009, 27 (03) : 275 - 280
  • [5] Simple SPION Incubation as an Efficient Intracellular Labeling Method for Tracking Neural Progenitor Cells Using MRI
    Chen, Chiao-Chi V.
    Ku, Min-Chi
    Jayaseema, D. M.
    Lai, Jiann-Shiun
    Hueng, Dueng-Yuan
    Chang, Chen
    [J]. PLOS ONE, 2013, 8 (02):
  • [6] Highly efficient and large-scale generation of functional dopamine neurons from human embryonic stem cells
    Cho, Myung Soo
    Lee, Young-Eun
    Kim, Ji Young
    Chung, Seungsoo
    Cho, Yoon Hee
    Kim, Dae-Sung
    Kang, Sang-Moon
    Lee, Haksup
    Kim, Myung-Hwa
    Kim, Jeong-Hoon
    Leem, Joong Woo
    Oh, Sun Kyung
    Choi, Young Min
    Hwang, Dong-Youn
    Chang, An Woo
    Kim, Dong-Wook
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (09) : 3392 - 3397
  • [7] Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale
    Cho, Myung-Soo
    Hwang, Dong-Youn
    Kim, Dong-Wook
    [J]. NATURE PROTOCOLS, 2008, 3 (12) : 1888 - 1894
  • [8] In vitro neurogenesis: development and functional implications of iPSC technology
    Compagnucci, Claudia
    Nizzardo, Monica
    Corti, Stefania
    Zanni, Ginevra
    Bertini, Enrico
    [J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2014, 71 (09) : 1623 - 1639
  • [9] Epidemiology of Parkinson's disease
    de Lau, Lonneke M. L.
    Breteler, Monique M. B.
    [J]. LANCET NEUROLOGY, 2006, 5 (06) : 525 - 535
  • [10] Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation
    Doi, Daisuke
    Samata, Bumpei
    Katsukawa, Mitsuko
    Kikuchi, Tetsuhiro
    Morizane, Asuka
    Ono, Yuichi
    Sekiguchi, Kiyotoshi
    Nakagawa, Masato
    Parmar, Malin
    Takahashi, Jun
    [J]. STEM CELL REPORTS, 2014, 2 (03): : 337 - 350