Cellulolytic Microbes in the Yanbaru, a Subtropical Rainforest with an Endemic Biota on Okinawa Island, Japan

被引：10

作者：

Fujii, Katsuhiko ^{[1
]}

Oosugi, Ayaka ^{[1
]}

Sekiuchi, Shiori ^{[1
]}

机构：

[1] Yamaguchi Univ, Dept Agr, Yamaguchi 7538515, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 05期

基金：

日本学术振兴会;

关键词：

cellulolytic microbe; Okinawa Island; sub-tropical forest; cellulase; xylanase; SPECIES IDENTIFICATION; SOIL; STREPTOMYCES; CELLULASE; TRICHODERMA; MICROORGANISMS; ALIGNMENT; ENZYMES; GENUS;

D O I：

10.1271/bbb.110881

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellulolytic microbes in the soil of the Yanbaru, a subtropical forest with an endemic biota, on Okinawa Island, were isolated and characterized in a search for novel microbial strains with biotechnological potential. Soil samples of the Yanbaru were suspended in sterilized water, inoculated on mineral salt agar overlaid with a filter paper as carbon source, and cultivated aerobically at 30 degrees C. After 2 weeks of cultivation, emerging colonies were isolated and subjected to phylogenetic and enzyme analyses. The phylogenetic analyses revealed bacterial and fungal isolates belonging to nine and three genera respectively. All isolates possessed cellulase activity, and several strains showed strong activity comparable to Trichoderma cellulase. Many isolates also exhibited xylanase activity.

引用

页码：906 / 911

页数：6

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