Resvega Alleviates Hydroquinone-Induced Oxidative Stress in ARPE-19 Cells

被引:26
作者
Bhattarai, Niina [1 ]
Korhonen, Eveliina [1 ,2 ]
Toppila, Maija [1 ]
Koskela, Ali [3 ]
Kaarniranta, Kai [3 ,4 ]
Mysore, Yashavanthi [1 ]
Kauppinen, Anu [1 ]
机构
[1] Univ Eastern Finland, Sch Pharm, Fac Hlth Sci, Kuopio 70210, Finland
[2] Helsinki Univ Hosp, Dept Clin Chem, HUSLAB, Helsinki 00290, Finland
[3] Univ Eastern Finland, Inst Clin Med, Dept Ophthalmol, Kuopio 70210, Finland
[4] Kuopio Univ Hosp, Dept Ophthalmol, Kuopio 70210, Finland
基金
芬兰科学院;
关键词
hydroquinone; Resvega; retinal pigment epithelial cell; ROS; ARPE-19; cell viability; inflammation; antioxidant; NF-kappa B; NADPH oxidase; RETINAL-PIGMENT EPITHELIUM; NF-KAPPA-B; MACULAR DEGENERATION; REACTIVE METABOLITE; INFLAMMASOME ACTIVATION; BENZENE; INTERLEUKIN-4; PROTECTION; SMOKING; INJURY;
D O I
10.3390/ijms21062066
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Retinal pigment epithelial (RPE) cells maintain homeostasis at the retina and they are under continuous oxidative stress. Cigarette smoke is a prominent environmental risk factor for age-related macular degeneration (AMD), which further increases the oxidant load in retinal tissues. In this study, we measured oxidative stress and inflammatory markers upon cigarette smoke-derived hydroquinone exposure on human ARPE-19 cells. In addition, we studied the effects of commercial Resvega product on hydroquinone-induced oxidative stress. Previously, it was observed that Resvega induces autophagy during impaired protein clearance in ARPE-19 cells, for which it has the potential to alleviate pro-inflammatory pathways. Cell viability was determined while using the lactate dehydrogenase (LDH) and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, and the cytokine levels were measured using the enzyme-linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) production were measured using the 2 ',7 '-dichlorofluorescin diacetate (H(2)DCFDA) probe. Hydroquinone compromised the cell viability and increased ROS production in ARPE-19 cells. Resvega significantly improved cell viability upon hydroquinone exposure and reduced the release of interleukin (IL)-8 and monocytic chemoattractant protein (MCP)-1 from RPE cells. Resvega, N-acetyl-cysteine (NAC) and aminopyrrolidine-2,4-dicarboxylic acid (APDC) alleviated hydroquinone-induced ROS production in RPE cells. Collectively, our results indicate that hydroquinone induces cytotoxicity and increases oxidative stress through NADPH oxidase activity in RPE cells, and resveratrol-containing Resvega products prevent those adverse effects.
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页数:13
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