Impact of ITS-Based Sequencing on Antifungal Treatment of Patients with Suspected Invasive Fungal Infections

被引：6

作者：

Guenter, Sara ^{[1
]}

Gorkiewicz, Gregor ^{[2
,3
]}

Halwachs, Bettina ^{[2
,3
]}

Kashofer, Karl ^{[2
]}

Thueringer, Andrea ^{[2
]}

Wurm, Phillip ^{[2
,3
]}

Zollner-Schwetz, Ines ^{[1
]}

Valentin, Thomas ^{[1
]}

Prattes, Juergen ^{[1
,3
]}

Wunsch, Stefanie ^{[1
]}

Ullrich, Elisabeth ^{[1
,4
]}

Zurl, Christoph ^{[1
,3
,5
]}

Hoenigl, Martin ^{[1
,6
]}

Krause, Robert ^{[1
,3
]}

机构：

[1] Med Univ Graz, Dept Internal Med, Sect Infect Dis & Trop Med, A-8010 Graz, Austria

[2] Med Univ Graz, Diagnost & Res Inst Pathol, A-8010 Graz, Austria

[3] BioTechMed Graz, A-8010 Graz, Austria

[4] Med Univ Graz, Inst Hyg Microbiol & Environm Med, A-8010 Graz, Austria

[5] Med Univ Graz, Dept Paediat & Adolescent Med, Div Gen Paediat, A-8010 Graz, Austria

[6] Univ Calif San Diego, Dept Med, Div Infect Dis & Global Publ Hlth, San Diego, CA 92093 USA

来源：

JOURNAL OF FUNGI | 2020年 / 6卷 / 02期

关键词：

invasive fungal infections; ITS-sequencing; antifungal therapy; PULMONARY ASPERGILLOSIS; DIAGNOSIS; CANDIDEMIA; DISEASE; TESTS; BLOOD; SERUM;

D O I：

10.3390/jof6020043

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Molecular techniques including the sequencing of fungal-specific DNA targets are increasingly used in the diagnosis of suspected invasive fungal infections. In contrast to established biomarkers like galactomannan or 1-3-beta-d-glucan, the clinical impact of these methods remains unknown. We retrospectively investigated the impact of ITS1-sequencing on antifungal treatment strategies in 71 patients (81 samples) with suspected invasive fungal infections. ITS-sequencing either confirmed already ongoing antifungal therapy (19/71 patients, 27%), led to a change in antifungal therapy (11/71, 15%) or supported the decision to withhold antifungal treatment (34/71, 48%) (in seven of 71 patients, ITS-sequencing results were obtained postmortem). ITS-sequencing results led to a change in antifungal therapy in a relevant proportion of patients, while it confirmed therapeutic strategies in the majority. Therefore, ITS-sequencing was a useful adjunct to other fungal diagnostic measures in our cohort.

引用

页数：8

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