Iron fortification of rice seed by the soybean ferritin gene

被引：491

作者：

Goto, F

Yoshihara, T

Shigemoto, N

Toki, S

Takaiwa, F

机构：

[1] Cent Res Inst Elect Power Ind, Dept Biosci, Chiba 2701194, Japan

[2] Natl Inst Agrobiol Resources, Dept Biotechnol, Ibaraki, Osaka 3058602, Japan

[3] Hiroshima Pref Agr Res Ctr, Inst Biotechnol, Hiroshima 7390151, Japan

来源：

NATURE BIOTECHNOLOGY | 1999年 / 17卷 / 03期

关键词：

nutrition; iron content; genetic improvement; glutelin promoter; Oryza sativa;

D O I：

10.1038/7029

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To improve the iron content of rice, we have transferred the entire coding sequence of the soybean ferritin gene into Oryza sativa (L. cv. Kita-ake) by Agrobacterium-mediated transformation. The rice seed-storage protein glutelin promoter, GluB-1, was used to drive expression of the soybean gene specifically in developing, self-pollinated seeds (T1 seeds) of transgenic plants, as confirmed by reverse transcription PCR analysis. Stable accumulation of the ferritin subunit in the rice seed was demonstrated by western blot analysis, and its specific accumulation in the endosperm by immunologic tissue printing. The iron content of T1 seeds was as much as threefold greater than that of their untransformed counterparts.

引用

页码：282 / 286

页数：5

共 42 条

[11] THE EFFECTS OF ORGANIC-ACIDS, PHYTATES AND POLYPHENOLS ON THE ABSORPTION OF IRON FROM VEGETABLES [J].